XB-CLONE-3559227
Clone Name: IMAGE:7711974
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Gene:
prmt3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX969164 [+]
ctgagaccagtttattccacatcggtactgtataaaacatatctgggctactaggtactaaggctggcagggattggctactggagggcatatgtgtggg
cggcgccgtttaatagaagggtaataatgagggagcgcggatccttccggttcttgcgcacagttattctgccttccaacacgtccccggctttggcagg
gatgggcttctccagcataaaaaccgtctgtttccagtgcgttttggtgcacgaggggctcgtggagaaggaaaccgctctctcgcagctcctctcaaag
caaacgtcaaagtaaccggcgagtgccgtgcaaggtgcatccctggttacactcagggagaaatccgaagcaaaatctaaatcctttatggttgctgtct
gacagttgatgttctttatgatagaaggttccgaaatctgcgtctccgctttcactacttccacgacggcttcgggaatgacgcatttcttcatgcagga
catgttgaagccgtacacgttttcccagaatgcaatcttccccgcgtgcttcgtctcgtcgctcagggccaccaagctcatggtacaggtatcggggtac
actgccccgtcctcattcaggtacttgtctctggcacagataaccgaatccaacatggactcaaacaggaggaaatagcccatccattccgatatgataa
tgtcgaccttctccacgggcagatccacatcttctactcggcctttaaccagagatacggtatcttccagcccattcagccggacaatgtccatggcctg
atagataatgtctgactgatc
3' EST: CX969163 [+]cgacgatgcagcctgggaggatgaggaggggatcgctgaacccgacgtgcgctgtctgttttgtgaaaggctctccctttcggctgaagaaacattttct
cactgcaaatcccatcatcagtttaatattggctccttcatgaggaaacacggccttgacttctacggatacataaagctcattaactttgtcagagcta
cggggggcgctgctgagtctctgagtcacgtgactgcgccgctgccttgggataacgatcagtacatgattccggttgtaaaggatgatcctctccttca
gtttgatgtggaggatctagaagactgcgccaatgagtcgccgagtgcgggggagccgtccatgtttgagcgctgccggctggcagagagccgagctcag
gccgcagagacaaatttatccttggccctggaagatcttcacaaaatgagacaacttgcgcaggattttgtgatgaacgcagatgtcagacacagcccgt
cttccgcaggagccatcgctgacctggatgaggatggtgtgtacttcagctcctatgggcactttgggatacatgaagagatgttaaaggacacggtgcg
gacagagagctacagggatttcatgtaccagaaccctcacatattcaaggacaagacggtgctggacgtgggctgtgggactggaatcctctccatgttt
gcagccaaggcaggagctaagaaagtgattggggtggatcagtcagacattatctatcaggccatggacattgtccggctgaatgggctggaagataccg
tatctctggttaaaggcgagt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: