XB-CLONE-3558971
Clone Name: IMAGE:7712381
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Gene:
rnpc3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX969341 [+]
agaagagcggggcgcaaagtgtgcgcctgggagccgggcggaattataagctgagaactactgcattcgctacctatccaaatgaatttgcagcagcaaa
ggctctgtctgtacttcaccaacttaccatcttggggcacactctcgtggttgaatatgctaaagaaaatcacattcacttatcagaccagcctcctttc
tctgaaaaaaagaaaagtgcagagaacaaagatgatgcgaaagacaagactcctccggatcagatatctatcgaaagaggaatagccccaaatcatggac
ttctctttccaataaagtccagtctaaagtatctgtatcctccaccaagcagcactattctggcaaatattgccaatgctttggcaagtgttccaaagtt
ctatgttcaggtgttgcatttaatgaataaactgaatttaccagctccttttggaccactaacagctcagcctccagtatatgcagactatttagcacta
cctatgccatacccacctgtgccaccagaccttcctgaaaacccccctttgccagagctcgatgatgattatgacatggaggtgtccagcagggaagagt
cggaatatgagagtggggaagaggaggataaagagagaatggctcgtctgaaggaaatagcaactttgctgcctaagagaaaaaatcaaaagaagcaaag
ccagccacgaaaaaagaaaaagttaaaagattatattactgtgcctgctgtgcctcatagcaatgttcacacaccattacagccttctgatgtatttgaa
gaaccatgtattatgggcccaaaaaaactggaactgcatattcctactgacattctaacagctactccagagccagaaa
3' EST: CX969340 [+]gaaaaataaaatttatttacactgtaaaaaatgtcacaaaatatgtacattccagaagacaggcatttcataaatagacatttttcagtgttacatgtct
gcagtaaatatgctgttcctcttgatgagctttaccatatagaacttaaatggctgcatccaacctttggttaaatataacagcaactgtccaatatgca
tacaagatgctagaactcagcagcaggtataatcatgtatatgcccctacctctgctgtatgatgaataccgctgtgcttttaatatgaccttatgcctt
ctcaaatgaatggaagataaatagtatatctctaaaagcaattaacactttaaggggttgttcacctttaaggtaactgttagaatgttacagaatctcc
tattcctagaaacgttgcaattggtctttattattattatttacaatttatgtactatttgtcctcttctgtctatttcaagctttgaagtgggggtcag
tgaccccggttgccaaatgtgactaaaatgttattataattgttactttttattgcttaacttgtaattcagtgtctcttctattcatattccagtctct
ctttaaataaaaaataaatgtagagtattaccagaaagtaaatattgatgaaaatatttcctatacatgggtaagatatgacagtggttaggttctgcac
ttaacctttttattatatcatgtttcatgatcagcacagccattttgccttctccaatgtttgcaccattttagcactaacnataaatacataaaggata
ccacaataaaggata
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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