XB-CLONE-3558949
Clone Name: IMAGE:7712431
|
Gene:
chga
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX969357 [+]
ctctccctgggagccatctccatagccctgtgtgcggcacaagtcatatcattcccactatcagaaccagccagtgacaatgatacaaaggtaatgaagt
gtattgtagaggttatttcacactccctgtccaaacctagcactgactccattagtcaggagtgcctggagaccctcagaggagatgacaggatcatttc
tattctccgacgacagaaccttttaaaagaactccaggaacttgcatctgaaggaggaacaaagagggagcagaagaagaaaaaaaatggtggctatgaa
gatgaactttctgaagtccttgagcggcagaataataaacctgtgcattctgaaaaatctggtgaggtgtcttcggaagatcagaagtctctaagacctg
agcagtcagcagagagtgaggagagcaagaggcaagtagaagatgatggggcgaggaaggctgatttacagcaggaagagaacagtcaggaagatgatgg
tgctgaagagctggagagcaatgagatcagtaaacgggaagaggcccctggtgatgaagaaatagacaaccgtatcacagatgaaatcaatgaccaggat
atacctacagaaaagcaggaagataccagtgtggcgcatacttctgaggaggcaatggataaatccagactgaaagatgcagaggaaatggcatcagatg
agagaagggactttgagaaagacaactcanaggaagatacaaacgaaacagacgaagaagaggatgagacccagagagaagacaactctatgaagcttag
tgaaggtcaagagtttgacag
3' EST: CX969356 [+]gtgaagaaaaatatcctttattttcactttttggtatgttgcttaacgcgacattctaacaaaagatcaattcattctttaattccttctttttctctag
tagacacaaaaagtccaatttagtgaaacatgtcgtttaagatgctgttctccaatttgagatcatgtattataatgaatataggctgaagctactgtac
tagtacatttaataaatacaagttgttaaaaaggtgaacttctcttattgagtaaaatatttatgaagcgagaatccctataaaagattgttatatgtaa
tacagcttgacatggaattggcagctctaattcgcagtttgcccatcctacatagcacctgtttgaacaaagaagaaagggatactaatagctgccaaaa
caaacagcagacaattagaagccctttaataggcctacctgaaatattctcattccttggctttaatctctagcagtttcctgcttgtgggctgtacttc
accccaccctttcagtaaaggttcagtcagggctattagggattgttactttgcaaagatatcaaaagatagtttttaatcacaaaaagcagaggccaga
aaaaaaatgcacctgcaaatacaaatgtgccaactctgcttgtctttggcagtgaaaaccagagggtgtaacaaggtaaatgcagccatacatacagggg
cagatttactaaaacatttctcatttttttccttttgaaaattaaaacaaactcatttcactcatgaatgtctgatatttacttaaaaagttgcaaaaag
taataaaactccagattt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: