XB-CLONE-3558901
Clone Name: IMAGE:7712245
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Gene:
XB5857568
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX969394 [+]
atttggatgaaggatgaacctaagagagagcctggagtagcagaggcgcttgtgactaatataaaaatacttgcagagcattatgtacgctacttggaaa
acactgagattggaaaagaactgggaatcaaagaagatatagcttctatgaaagaaagcattaatgagttgaaaaaggagattttaaaaatgacaaagga
tgcctgggaagaggttgacaaggaactaaatcagaagtacccaattttcacaacaaaagtcctcccagctctgaaggacttcaagcaacgctgggataaa
catatgacagcattagcaaaagaactagaaatcatgagtcacaaactattttccaatgtcaaaaaaaatttggatagtttttttgatacgctcagctctt
ctgcctcacgtggtcgagacaagctacgcgatgaggtagagcatctgcgcaccactctgctcccatacgcagaagatttccgcaaagagctagagaaggc
caaatcggaagtgaatgatgacgtagaagaagtaagaaagtatatataccagggagtggatgagatcagagagcagataaaaccctattttgacaaacta
aaatctgaggcgaatccacatgcagaagagttgcagaagcatttgctcaagttactggatgatattaaagaagctactggcataaaagcttaatcaacca
atgccatcaagtaacagtgaaagaataaattattcatttgctcattcacagttgagcttatacactgcaataactcagaattgtgctgatactcctccca
ttaccaattcttcattaattatcatttctgcttagaaattagctacaaaatcttgccatttgcctactaggaaatccctaatagtttccctctttcccta
ctttcta
3' EST: CX969393 [+]aaacaaaaaaaaattttttttttaaaaaaaaggggggaaaagggaaaaatttttgggtttcccaaaagggaaaaggggaaatttttggtcctattttcaa
aaaaaaaaggaaaataaaagaaaaatggggaggggggggggcccccccccattttggggttttgggggggaaaaccccccccggggaaggaaaaaaaaaa
aattttttccttccccgggaccggggggggttggggaaaaaaattttttagcccaatgttttttttaaaaccccccaaaaaatggggaaaaggccccccc
caccctccgggggggggcccccccccaaattttttttggcaaaaaaggggttttttcggcccctgaacccccccccccccggggaaaaaaaattttttac
cccctcccagggaaaaattcatttcgaattggcccccccccaggctttggggaaaatctccggggaagggaaaaaaaggggggccaaaaacccccccccc
cccggggaattggcccccccccgggggggaaaaaaaccggacccaaaaaaaaaacccccccaattttttttggaaaaggaaaaaaagttggggaccccga
attcctttttttttggcaaagggggaaaaagtt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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