XB-CLONE-3558797
Clone Name: IMAGE:7712255
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Gene:
trmt2a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX969470
[+]
aacatctgcatctctgaagaaataagtcaagaaggttccccagcagaggaagatggaagcaatgaaatggcaaaatcttctgctgagtcctgtccagaca
gcaagaataaagcagggatttatagatacatcaaagacgatctcttcacttcagagattttcaaagtagaaatacaaaacttgccaaaatacatatgctt
taatgacttaaaaaaattccttggcaagtatgggctaaatcctcataagattaaactcatgaataaacaaacttttgcatttgtgacttttaaaagtgaa
gaagagcgtgacaaagctatgaaagtcatacatggggtgaagtggaagaaccgtacacttagtgttcgacttgcaaagcccagggctgaccccataatgc
aaaaaagaaagcaggaagaaggagatggtgaacagccagaaagcaagcgtcaggctgcacacaatgatgcagaggaggagcccctcagtaaaacgatctc
agatgtagtgactcctttgtggcaagttccatatgaagaacagctgaaaattaaggaggaagaatgttgcaaagtattgaagcagttaacaaagagcact
ccatatgatgtctatagccctgaaacatatgccggtcactggaagcagcttactgtccgtaccagcagaaaggggcaggttatggcaatagtgtatttta
acccccagaaactgcctaaggaggatctctcagagctaaaaacgtcactatcaatgtatttcactgaagggtcgggggaggaaagt
3' EST: CX969469
[+]ttaagtggacacactaaacacatggaaatgctgttggttggtccagttttacatttaaataaagtgactcaagagagaactcattagttacaacactgaa
atgaataacaccatgaatctttgccctcactgccgtaccttcggcagctttactttccataacaacctcagtctggccgaactgtgtcacggcacaagtg
acggctcggtgcttttagacggatcagttacgatttctgcaggttccgttctctcaaacagaataaccaattcaaaatgtggagtctgcgggaaaaggtc
tacagctaaggctttgattggccgaaatggtctccccttgacacgattggaaggagcacggcacaggtcaataaagttgttcattgctgccttgggattg
caggaaacatatatcagttgcttcaaatgctctgctttcctaattgcaataatcaccttggagtggaggccggctcttggtggatcaacaatggccagtg
ggcttgggaacgtgaaagagtaaataagggtgggaaagatgtcctcagctttgccacatcggaactctacattgtccaaattatttagttgagcatttgc
cttggcatctgcaatagcctcctgacacagttcaattccaacaacttttttgactttcttagccaaagaaatgccaatggttccagtcccacagcaaaca
tccaaaacagtggtgttctgatccagctgagcccagtctgcaatggctgaatacagaacctcagctgcaggtgtattaacctggaagaaggcatgaggtg
aaattctgaaggtagaccaagt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: