XB-CLONE-3558788
Clone Name: IMAGE:7712399
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Gene:
cox20
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX969476
[+]
aatggaatttcttcatgctgggcagtccatcttcagctttatctgggtttctgttgaattagttcttttcataatgcaggtttttaagctgtccaaccat
ttagcaaacagaaaactgccatttctggctcagagtgcaggaatcagaaatgaaatgtgagcgcatacttacactatttccctagttagtggcaatcaca
atcaagactacatggcatcaagcagttgtgttccctattgaaattttttctgcttcattcagtgcccattgagcaaactactcattccaggcacaccttg
gtttgtacacatttgtcagtatggcctgctattcttggggtttattagcatttggcacttagatgtacatactaaagatgttggtggtgtatatacaaga
acagcattcattgactcaaacaagcttagctattctaagcttaccatttgtttaaaattaagttaaaagttttaaagtattaaaagttttttggcagaat
caataacagtatttttgttttaatttgcaggatgcactgcagatacaataatgcaaaagtaaggatccagcaaaagatgttacaagaaggaattaaaaat
aggatactatttgaaggcagcagtatagatccaaatacgcgtaaaaacacaactgactcaaaaacctgattttccgcatcaaaaacaggattccaatgca
tatgctaaacacctttccaaaacctgcatttaaagaatataggagtttgtggcagtaacaataaaccacaccttttcagtaactgttaaatgaccaccac
ctttatattttttttctataaaatatgccctgtggcgttgacaattgggtacagcactacata
3' EST: CX969475
[+]atagatgctaatatggtccttgcacaaaaaaagattagtctaagaggagaaggggttacatgactgaaggtgtgggaaaacagtgatattaggagtgatt
gtagtgaaagatctggtttctagttttgtacttgggaggtggcatatggtatgctaaaagaaaacagattagatggtagtatggctctgtaaaaattgtt
aaaatgttagtctgcgagattaaatgtagagggtatttctaaggaagggggtgcagctctggtgacatttttaatttgtttgtgcaatgcaatgaggagt
gaatagtgcatcaaaatatgttgatgtagctgagtggagattgcaagggttagtatttgaattcatcctgaaaggaagcctgtgcatggcagaggaggaa
tggttgatgaggtacataacgggtgtagtgactgtctggagaggaaagtaaatttaatagaaagttgcagtggtgatattacaaaatataaatttctgtg
caaatttttacaaagtgactaattaaaattaatgttgtagctcttctgaattgatcccaaatatgcaaattattactcaattaagtggaaaaaatctaaa
gcacgtaaaatttttattgctgacacttcaacccctgcaaatgcaataaatttgttaaatttaagaagagttataaataggtgtgtttgtgggaaaaatc
aacttaaaatttcagacttgcagcattttctgtatcatattatgaaaagacatttgttgagacacggcattcatttttattaactacttgtctgaaactg
catatacttgtccataccatgaggtatgtgtagccccctaaatgagtactgcattttatgtt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: