XB-CLONE-3558748
Clone Name: IMAGE:7712355
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Gene:
eif3e
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX969502 [+]
gcatttggtcttccctctccttgaatttctatccgtcaaagagatttacaatgagaaggagttatttcaggggaagctggacctcctcagtgataccaac
atggttgactttgccatggacgtatacaagaacctatatgcagataaagaaatccctcacgctctgagggaaaagaggaccacagttgttgcccaactga
agcagctgcaggcagaaactgagcccattgttaaaatgtttgaggatcccgagaccactcgccagatgcagtccaccagagatggccgaatgctgtttga
tcacctcgctgaaaaacatgggtttcgacaggaatacttggatactctttacagatatgcaaagtttcagtatgaatgtggcaactattctggagctgcg
gagtacttgtactttttcagagtgctggttccatctacagacagaaatgccctcagttctctctggggtaagttagcgtcggagattttgatgcagaatt
gggatgcagccatggaagatctcacaaggctaaaagaaacgatagacaataatacagtcagttctccactccagtctcttcagcacagaacatggctaat
acattggtctctgtttgtattcttcaaccacccaaaaggaagagacaacatcattgacttgttcctctaccagcctcaatacctgaatgccattcagact
atgtgtccgcacattctccgatatctgacgactgccgtgatcaccaacaaagatgtgaggaaacgaagacaagtcttaaaagatttggtt
3' EST: CX969501 [+]aagggcgagaggcttttgaagaaaatgctatgtatttattatattaatatggcagtgggaaataaatttgaagagccccgtacaataaatgtggtacatg
agtactaaatcagaaaaaaaaaaaaaaataaaagcataaaataaccaataagtcccagttaaaaggaagtgtacccattttattcaaacgcatgccttcc
tttaaccttacaatgtggcttggtttttgtaagaggaaaagaaagcatgggtaacagggaaactttgagctggtgtgaagttcaaatgaagtcatcaata
gaatccggaatcctgagctgcccaggtaggtgcctcagttctgctgttctggttcgttttcttctcaatattcatagccagcatttggctacggaatgca
aggctctttgtcttttcaatcacttgctgataaggagagacggcattgtttcccataaccacgtgacccagtttggaatctatcttggcgtccagtcttg
cattacgaattaagttgacaatccacctttcagcttcttccggagtcatattcagtttgtcagccaacatgctgatgcttatacactgatggatgcgaca
gaaagtctcaaatataaagagtctggcattttcaatgaagtcttctaggcaggccacaaggaagaagtcatttaccagcaccgattcgcattctcttaac
tttttctgtgctccatcaaagtcaaagttgacatataagcattccacaaactcagtaatcgggtctttgtaagtgtaggattcctgttggatgaccttaa
cc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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