XB-CLONE-3558567
Clone Name: IMAGE:7712421
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Gene:
sult1c1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX969633 [+]
acaccctgactgcagtatggatccagcagtgatgaaacaactggcaaaggaaatggagaattaccaggttattatgggccaagttgagggggtccccctg
ccggaccccacctgtgacgcgtgggactctatatacaatttccaggcccggggggacgacattctcatcgctacctaccccaaagcagggacaacgtgga
tgcaggagatcgtggatctgatattacaggagggagatgcgcagaagggcaggagggccccgacttacatcaaagtgccattcatagaattgatatcccc
aaagcccatgccgtcaggtgtggagttggcacaaaccatgaaatccccccgtgtcctaaaaactcacttacctattaacctgctgcccccctccttttgg
gagaagaatgtcaaggctgtctatgttgccagaaatgccaaggactgtatggtttcttattattactttcataagatgaatatatttttgccggaccccg
gaacctgggagaatttcttctcgacattcctgtctggagatgtgccatggggcagttggtttgaccatgtgattggctgggggaaagctatggacaaaca
ccagattctctttatcttctatgaggacatgatcgaggacccaatgcgtgagattcgcaaagtcacgaagttcctcgggaaggacttatctgatgaggtt
ctggagaacatcaagtatcacacttccttccaggcaatgaaggagaaccctatgactaataacagcacagt
3' EST: CX969632 [+]ggttttttttttttttttccatgagaggaactttatttcagtagaaaaatatcagacaatgggtttgaggcttcacttatctaagacacaataggcagat
ctatactttggcccaacaggttccttatggcccgtacagaggcacaaaggcgccaccaggaaagggagtcgtaggagaatctcttattgggctccatgtg
cctctggctcctctgtacaatcttgtgggcacaggttgtcccctgtgtgaagatgaggaagggaattcccaccggctccttaggccaaattctctatcag
agttcagtgcggaagttcagcccactgccctccatcttcttcttgtattcctcatcaaagataatgttctgtgccaccgtgaaatggtttttccaatccc
ccacggtcccttttctcaggaaaggagatatggtttcatccatgatagaattgggaactgtgctgttattagtcatagggttctccttcattgcctggaa
ggaagtgtgatacttgatgttctccagaacctcatcagataagtccttcccgaggaacttcgtgactttgcgaatctcacgcattgggtcctcgatcatg
tcctcatagaagataaagagaatctggtgtttgtccatagctttcccccagccaatcacatggtcaaaccaactgccccatggcacatctccagacagga
atgtcgagaagaaattctcccaggttccggggtccggcaaaaatatattcatcttatgaaagtaataataagaaaccatacagtccttggcatttctggc
aacat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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