XB-CLONE-3558547
Clone Name: IMAGE:7712375
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Gene:
rnf213
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX969646 [+]
aaaaacctagaacaagtgaagaactccctaggggaaagcgtggataatacggccactgtactccatcttgttctctgcaggatgctcactatttccatta
atcaacctgaccagcgtcttttgccttttgatcatactctgtcgacaaaggacgtgagaagcaactgggagagaaatatggcggcatcggtcattgttcc
tgtgcttacgaatttaaaccatgatcttggtgacgtcaataattacattaggaacgatgagagaataagttccgacccaataatcagaatcatatatggg
gatccgttgcgtaacaatgatattgtggcactgccaatacatggactcattccctaccccaaaatatggagctgcagggcaagaatcactgttgggcatc
ttcagcatttggttcagcagagggatggggggaacaatctgccaatgctgtcaacgtttctggagaatgaaagtgaactccgcctggttaagttcctacc
agaaatcttaaatcttcaaaaagagttggtgaaaagattccagaatttccgagatgtgagcaaccagacaattggggaatttttagccagcataactgtt
gatggtgctcgacgtgtgtttgagaagagaatacaaaccttcctatttacatggaactgtctaagaaattctcttcttatgaaaggggaaataaaactcc
cagaagaagcctgtgccaatgaccttaccccagcgagtgattttgccattctgcttcctagtcggcaaggacagggtctctgtgccacagccttggtcag
ctatctcatcgctctgcacaacagcctggtttatgctgtgaggcg
3' EST: CX969645 [+]gatagtacagaagagagtatttaaacataaagataacaaccataatacatcttcttactggttactagaatcattgaccccagagaccagaagatatata
catttcagatataaaagtaaaaagggtattattttgattattgcattctattgttaacttggatgtgtttgtataacatcaatggcgtttccacttcatg
gcggccgccaccttccaactctcggcacagtgagccaaacagatctgttctgggaaatcctcctccagcgccgggaatggttgctctttcccatcaagca
atgggcccagaacgtctcttaacccccatgcaggtgggaaatcattggccccacgttgcttcttgagctttaagatgatcgtttcatgtaattccagaag
gaagacattgagtccatgttgcgccaagaacgcagtcaggagcttctggctctcctcatctaattcctgcttatacccttcatccagatctgcaaatgga
tcgtttttaaggcgtatgcgatgctcggactgtaatgcggtgagaagctgccatagtgagattgtgtgcttcaactgacactgcttcagcgcctccagga
tgttctcttcgctctgatccttcatctgtaggacatcttctacataagcggtcagcgggagatctggattgcccccagacgttgctaagaaccccaacat
tgtctcaatgatttgaagcgcatcgcagacatcgctgtacaaatccagatctttgcttatgttttctatcaccgaatctagcagaggttcctgtaagact
tttgccctcacatctctaaatatgttctc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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