XB-CLONE-3558467
Clone Name: IMAGE:7712336
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Gene:
ankrd13c
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX969703 [+]
aaaatgtataaaatagaaataaccttctgtagataatgaaccaaatactgaattaatatatggttgccaactcaacctattgcaatagctgttagagatt
aaatgatccagggacactatgaattcacagagcagtgcagaatcatgtagtgctcctttaacttttttttttttttttttaaggaaatttttattttaag
taaatgttcttcttgtatatctgtatctataatatttcagtttatttgctctttaaaagcaaaatctcccatgcctactatacggtttcaatctctggga
aacttttcttattggcaggagtgggaagtgcctttgctctgctcctgtgagtataaaatgaacatatgcctgggcacattgtcaggtggaatgggtgcaa
agcagttgccactggggagcaacttgcagtaatccactacaagtacagatcaatgaatctgaaagtgtgctttgacccttatttcttaattagatattat
tcactgcatcccatacaaagatttggaagcagtttatttcttaaagataacaaacagtgcattacctcagagattctgcattttgatgtttaaatgtgcc
ctttgtttattagcatgcatatgaggagtcactcgctatggaatgcagtaagctcttaccccatttattgctattaccattagatcaggtggggacagct
cccctgctgcattacagagtacatgcgactcaattcacatgaattagctgtggactgtgggacgtatgccttgcgtcgggggaagcacgcagcatcactt
gttaaactagaggaggtgcagcaatgtttagggattccctttatt
3' EST: CX969702 [+]ctttacaaaaatactgaaacaaagtttaatcacacagattgaacacagctgcagaaataaaacattcgaagcataagattaaaacaaggagaccttcatt
aaattagtactgggcatggtttcagcaaaaacattgtcttaacggctgtttactagttacaaaatgatcgagtctacaacgtccagtaaaaatacctata
caacaattgtgatcggaaacaatacgctcttaaatgcagggggaaccatattccctttaagaaaaaaaaaatatcagtgttttggaagaccagactgaat
tccccatcctgaatatcagtttatatgtagccatggtgcattatgtgtaacaaatgaatatatcttttaataaaattacatatattacatgtgtcctttt
atattttttttttagccctgtaagatctcagaacttacagaaaacaaatgcaggtcctgccagcacagaacaggaatacgacagtactgagtgatgagct
atacaacgtgtaccgattgctggtaagcagtgtgtttggtaaacatatcatggaaaaatgtagtctattctttaaaaaaagtgccatttccaggtatatc
acttatgtacaaaatcccagtgcacttgcaaaatgttaataagtcttgaacacagaagatccggcaggagagacacttaaacgcagaaacagaatgagga
gaggttacagaatatcagacgtctgttcagatggagaatgaggaaacggagtaattatgaaccaaggtcttcattctataatgagggagttaaattttta
atacatatggcgcaatcgctgccttcagtcatgcatcttgcctact
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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