XB-CLONE-3558434
Clone Name: IMAGE:7712196
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Gene:
sf3a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX969726 [+]
ctttaatcagctgtataacaattcgtacaattaggtctatttctaccaactatataggtatttattttagcaaggcaaaatgtatatttaaagatacttt
attttgctatacggagagcattagcatgctacaaatgcagattagattgcgtgcccagtttcccaagacagtttttttcactttcaactgccaaataaaa
tacagcaagagcagaattccatttaaggaaatttaaaaaaaaaattctatcaagtgagtaattcaaaaacaatgtcccaacctgatctacatttatacag
acaccaaaaatagactagcagccaatttgttctgttggccagatgggactgtacacatgttgtgttttctgcataaaaatggaagccatgcataataatt
gtgtatatgcacggctctgcaacatggcatgctggatattcattccatgtgttcacttcttccgccctcctctttctttcagggctaagtgaatggtggc
tccatttgtcatgttgtagtaggccaaagagttggaatccttaataaagataccatcatactgaagtttctgtttgccagctggcatgcctgtggcctca
tgaattttaacctttatgacagacacctgatcaggcagtggcagagtgaaagacagcatttgtcctcccagcttccattctgttttgtcttgcatgttgg
ggacttgaaccttgacagtaacaggacccttgttcctgcgtaggaactcctcctctggaattagcgtgtcctcactcttcatttttttggcagctggctc
atcctccagtgntggaggtgggtgagctgggggcattgg
3' EST: CX969725 [+]gggcaaagtttcaggaaagagagaagaaaaaagaggaagaagagaaagagagagaacgtgttgcctatgcacaaatagactggcatgattttgtggtagt
ggaaactgtggacttccaaccaaatgagcaaggtaacttcccacctccaaccactccagaagagctaggggctcgaatacttatccaggagcgctatgag
aagtttggtgagagtgaggaggtagagatggaagtggaatctgatgatgaggatgaagtgaagaacaacaaagcagaggattcctcctctcagatggacc
aagatactcaagttcaagatatggatgagggttctgatgatgaagaatatacatcaaaggctcctcttcctccagagtctcctatgcctccaccactgcc
tcctacaccagaccaggttattgtcaggaaggattatgaccccaaggcttcaaagcctttgccccctgctcctgctcctgatgagtatttggtgtcccct
attactggagagaaaatacctgctagtaagatgcaggaacacatgcgcattggactactggatccacgctggctggagcaaagagaccgtgctattcgtg
aaaagcaaggagatgatgaagtatatgctccagggttggatattgagagcagcctgaagcagttggcagaacgccgtaccgatatcttcggtgtggaaga
aactgccattggtaaaaagattggtgaggaggaaattcagaaaccagaggagaaggtcacatgggatgggcattctggtagcatggcacgtacccaacag
gcagcacaagcaaacatcacacttcaggaacaaattgaggccatccacaaagc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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