XB-CLONE-3558165
Clone Name: IMAGE:7712414
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Gene:
ddx47
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX969917 [+]
cgagagctgggagtcacagatgtgttatgtgaaacctgtgagcagctgggatggaaacagccaaccaagattcagattgaggcaattcctgtggccctgc
aaggtcgagatataataggacttgccgagactgggtcaggaaaaacaggagcctttgctttgccaatactgcagactctgctagaaagtccccagcgact
ttatgccttggttctcactccaaccagagagctggccttccagatctctgagcagtttgaagctcttggttccaccattggagttaaaagtgctgttatt
gttggtggcattgatatgatgtcgcaatcactggcactggcgaagaagccacatgttgtcattgccacccctgggcgcctaattgaccacctagagaaca
ccaaaggatttaatcttcgagctctgaagtatctagtgatggatgaggctgaccgtatcctcaacatggattttgagacagaggtggacaagatacttaa
agttatacctcgagaccgcaaaacatttctattttctgcaacaatgaccaaaaaggtgcaaaaattacaacgagcagctctgaaagacccagtaaaatgt
gccgtctcttccaagtatcagactgtggaaaagctgcagcagttttatgttttcattccttcaaaattcaaggacagctatttagtgtacatcctgaatg
agctggctgggaattccttcatgatattctgcagtacctgtaacaacacacagcgagtggccttgctactgcggaacctgggcttcactgccattccgct
gcatgggcaaatgagtc
3' EST: CX969916 [+]gaagaaatagaacttattttattattggtaagaaacagtttgagagtgtccaaagaaggcaccaggaaccagtttctagtggtgttacagcaattcattc
tggaattaatagaaaattttgcttggagttcctctgcgcatactgggatgcggctgtccttgagctctctgtgcaggacatgtaatctgcgttacaagct
gtacccatgatagcagatgtatagattggatgtccatgatccttaatgttcttttagccacaaagcaggaatcctgaacagtctttaaagttttttttat
cagtggatttttttctttttgaacttgccccctgtgacttttttccttacacctaatgacccctctttatcatcatccgcatcatcccgtgggcgccttt
tcttttctccatgttctctgagttcaattcgtgcaaatctctgtgcctcagaaactctttcattcagcatcatcacttcctcttcctgcgtaggaaatgc
cggaagcttctttccaatcaagtgttcgattcgttgaaacagctccacatcatactggctaacaaaagtgatggcttttccagatctccctgctcgtgct
gttcttcccactctgtgtatgtaatcctttgaatgggtggggatgtcaaagtttataacaacatctacatgagggatgtcaagaccacgactagcaacat
ctgtggcaagaaggatggatcgggactttgccttaaatttatttagcgaacccagccgcttgttctgactcatttgcccatgcagcggaatggcagtgaa
g
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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