XB-CLONE-3558090
Clone Name: IMAGE:7712230
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Gene:
slc34a3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX969971 [+]
tattgtgccatttatttcacaggcgctgtaagcaaaagaaccaaacgtgacaggatcttccccaaggataaagggcacggctaaaaccctgtcgtctgca
gctcactgctatgatttcctcttatgtttgggggcagaagcatgtgtttgcaggtgaaatacctatatgtgatttattatatatgctcttatattaccat
ggggcggagcttctcccatacgcccatacaagtcgctgtaagtcgcttagccctgaacagaaatctcattatcagttgttggggggtataaaaggggcaa
aaaaaaccctagcaccatctttgtaaaacggtaagttttcctctgtaaaatagccactggctagagctcctctccatcctgcctggaatcacattccgag
acgacccacctgagctacagataaacccatacgacttccactactggcatctgggctgctctcttcccagtgttcaaacaggaggacgttgatgtctgag
gaccagagagaacagtgtatggttgcccagtggaggttttttataattacagagatgtccagcttcgttctctggtcttcggtggaaaatattccatgtg
tcctttacgtcacaaccacagggaatagagcagccaatcagcaggacgtaaggaggaccacaagcccatacaggtaggataataacaagtttggacatcc
tacttggactccagtacccaaaggcaggatctgaatacccctttcaagctggtagtgaagcctttgttgctactggttggaaatccttggtttactaaga
tgcatattttgcctacttgntgatactttctgcccattccacagccttatgccaagtgatgcccact
3' EST: CX969970 [+]agaggtttcgccgggcatttggcggttctgctgtacatggcatttttaactggatgacagtcatagtgttgctgcccgttgagcttggcactggatatct
ctaccacatctctaaagccatcatcaacagtttcaacatcaatgcagggggacaagcccccgatatcttaaaagtcattacggagcccttcactcatctg
attatacggctggacagttcggtcatttccggaaatgccattggagatcctgagacaaaaaataagagcctgatcaagcactggtgcgtgacggagtcca
acacggtcacccaaaatgtctccgtgtccaacatgaccgagtgcaacatatttcagtgcttcgaaacagagaacggaacgttccgggaaatgaacatcac
tataaaggaaaacattgataaatgcacgcacattttcgttaacaccaacctctccgacatggccgtcggcttcattctactgacggcctccctgttggtc
ctgtgcgcatgtctcatcctcatcgtcaaattgctaaactctgtcctgaaggggcagattgctcaggcaattaagaagatcatcaatgcagattttccgt
tcccgtttacatggctaagtggatatctggccattattgttggtgcggtaatgacctttgtggtgcaaagtagctctgtgttcactgctgcgatcgttcc
attgatcggcgtgngggtcattagcatggagagagcctacccgttgttcctggggtccaacattggtaccaccaccaccgcagtgttggcggctctagcc
agtcctgccgatgccctcagcaattccgtgcaggttgccttcatccacctgttcttcaacctcacgggattggttctctggtacgtcgtaccattcctg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: