XB-CLONE-3558071
Clone Name: IMAGE:7712184
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Gene:
casp1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX969985 [+]
taaaagtgtactaacagattctgaagtggagtacatcaatgagtgcaatgccataaccaaggacagatgccggcgtatgattgacaatataaagaataaa
ggagattattctagcaacattcttcttcagtgcttggtggagaatcacaagacgctggccaaaaacatgggacttgatgtttcagaatcagctattgcac
cccaacccatactagagcagaataagaagacaaatgagaacacagtttcagaacctgctattgcaccccaacccatacaagagcagaataagaagacaaa
tgagaacacagaaatcagcagcgtgattcattgttcggataaagatttcaaggaaattcatgacactcaaggagataagatttatgaaatgcggaagcgt
gaaggacggaagcgcttcgctctaatcatctgtaatgaaaagtttgagactctgactgagcgccaaggggccaaggttgatctggatgggatgaccaaat
tactaaatgaattaggataccaagtccttcaagaaacaaacttggcaaaagaggaaatgctgaaggtgttgaaggagtttgcagcccgggaggagcatgt
ggattcagacagcaccttcatagtactgatgtctcatggagataagccaggagtctgtgggacagacagcaagaagatagaacatgaaaaaggacagtgt
caggtgactaacctgctacagacggatgaaattttttctatattcaacaatatccattgccccgaaactgcgggacaaaccaaaagtcattattattcag
gcctgcc
3' EST: CX969984 [+]ttacacaataaaaccttttttgcaattaacatttgttaatattgctcattgctttaaggcacagttaaaaagaaccatttagacgctatcatcatgttga
aacgatttaagagttacccgtaaacacccaaccttatcttgctgcagagaaaaagacattctaaattcaaaggtcttgatattcttggatccattgactg
ataccttacatcagtggcctgggaaaaggtaaaatttctttgtcagcgtactcctctcttgtgtgggcatctgcctttgacccttaaacgaggattgtac
ttcaaggaagatgtccatcaatggctggcaatgggctaattcattcatttttttaatcagacctgtgatgaacaaggaacccatggtaggatgtctccaa
gaaacagtatctggggttgaagaacagaagcaaatgaagtcagtctctctttgaactctatggagtgcatcatcttccaattgttcctctgggggcaaag
ccacagcatcacagactaatttttggcctctttcttccccacggcaggcctgaataataatgacttttggtttgtcccgcagtttcgggcaatggatatt
gttgaatatagaaaaaatttcatccgtctgtagcaggttagtcacctgacactgtcctttttcatgttctatcttcttgctgtctgtcccacagactcct
ggcttatctccatgagacatcagtactatgaaggtgctgtctgaatccacatgctcctcccgggctgcaaactccttcaacaccttcagcatttcctctt
ttgccaagtttgtttcttgaaggacttggtatcctaattcatttagtaatttggtcatcccat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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