XB-CLONE-3558068
Clone Name: IMAGE:7712280
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Gene:
faxdc2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX969987 [+]
ctttaatacattttatctgtgttttaggcaaaattagaacataattgtggcacacgagttagttgtgattagtgtaagaatatgatgctgttgcacccac
ccaatcccactcgtcactactgaataatgtacatacggtaacatccgtacctaatgggccactatggtgcacagtgatgtcagtgttctcaatggaccct
aaatctacagctaaatatccacagacactgggttttttttctatgcacctacagccatgaacaatttctaaccttctaattatcatttaatcacagagat
aattagtatcataagtcagtcaaaaactggagtggggggggggggggcggctccagtatttaaagccccaacacacccaaacccgctggatgttgctagt
acattagcacaacattaagggcaaaccatatccttgcaggtctctgttcaaaagggacaacttctcttctaacatttccccctttaaactattgggactt
tttaggggtgtcaggaatgctctgacttagtggtgtaaggttcattaataaaatgtgtctttcataagccttggtttgcttgaacatcaaatctgttcca
tgcagatgatccaataccccaagtactccgtagcactggttaaacttgaaatggtgaaaatcatggaactctggaaaaggcaggaaaggcaggtggtacc
cacagtgtgagatggtggtggtgatcaaagccaggc
3' EST: CX969986 [+]cggagtcaggatggccaggtgtgggactctgtaaagaagacagcttttgtcctgggcactgggcttctcatctttgctgcctttaggaattccgtgacat
ggcacttgcagcagttttggggagcctcgggggacttttggcaagcacagtgggggaagctgcacactttctttgatggaaatcaactggcgttgttctg
tttgggtaccatgctgatgcccacgctttccttctgggtcttcaatgccatgctgatgcttatcgacctcactggaaagccatacttcatcaccaaatac
cggatccagcctgggaagaacgaccccgtggatccagccaagctccgacaggccgtgatcactgtgcttgccaatcaggtctttctctcctttcccatga
tcgttctcatgtatcccatcatgctgtggcgaggaaatccctgtggccctgagctgcctacattccattgggtccttttggaactcacagtcttcgcgct
ggttgaggaaattctcttttattattcacaccggctggtccatcaccctctattgtacaagcgcatccacaagaagcaccatgaatggacggcacctgta
ggagtggtgtgcctgtatgcacatcccctggagcatattttttccaacatgctgccatccatggtcggccctatggtaatgggatcccacgtggccacaa
ccatgctgtggttctgcctggctttgatcaccaccaccatctcacactgtggctaccacctgcctttcctgccttctccagagttccatgattttcacca
tctcaagtttaaccagtgctacggagtacttggggtattggatcatctgcatggaacagatctgatgttca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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