XB-CLONE-3558005
Clone Name: IMAGE:7712525
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Gene:
fbxo32
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX970029 [+]
ccgaggcttacatgcagcctgtccatacgatgcaaacagaacattgtaaaaaataaattgcaaaatgcgcatctcacaaagtctcctgcaattattgcac
cagatatacagattaatgacacgaggcagcaaggaggaggaggaggaggaattcaaacactcaataaacactaggaaattcgtctgcaccaaatctgtgc
tctttacagggaaacagagtaaacaccagcaacttcagataaatatatatatttttttgcctaacacaggcctaatcagttctatgtagttctcttcatc
ttcatataatgatcccattaaaaaagtaccgttgttttgtttgctttatattcagcccctactgctctaggtgtttatatgtctggggtgtcccatccca
agctaacaatgtttttttccttttcatttcattcccagttgtctccccgttccataagggacttaaatattcacttcacttctaatgggcatatttatta
tagtgtgtaagtcaacaacatcgctgatgctgcccatagtaaccaatcagcaattagatttaaacagtcagctacaaataacgtttatacaatattgaat
tgtttaactcatccaccgcccctttagtttgcaggctggtgttgccttatggggaagccatagctgacacanaactacacatttggatcccgtacttctg
tgtatagtgattataggtgcagagctggctccacttacagtaaccctgagctgtccaactggaggcctgtgggccagatgt
3' EST: CX970028 [+]tcatagcagtcctgcgccaagatgtaccggcgccaaaggtgcaataatgatgtcatacagtaaagtctatgaagcctttaagcaaactgggggccataca
attttggagggcgacatctggcccacaggcctccagttggacagctcagggttactgtaagtggagccagctctgcacctataatcactatacacagaag
tacgggatccaaatgtgtagttttgtgtcagctatggcttccccataaggcaacaccagcctgcaaactaaaggggcggtggatgagttaaacaattcaa
tattgtataaacgttatttgtagctgactgtttaaatctaattgctgattggttactatgggcagcatcagcgatgttgttgacttacacactataataa
atatgcccattagaagtgaagtgaatatttaagtcccttatggaacggggagacaactgggaatgaaatgaaaaggaaaaaaacattgttagcttgggat
gggacaccccagacatataaacacctagagcagtaggggctgaatataaagcaaacaaaacaacggtacttttttaatgggatcattatatgaagatgaa
gagaactacatagaactgattaggcctgtgttaggcaaaaaaatatatatatttatctgaagttgctggtgtttactctgtttccctgtaaagagcacag
atttggtgcagacgaatttcctagtgtttattgagtgtttgaattcctcctcctcctccctcctgctgc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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