XB-CLONE-3557894
Clone Name: IMAGE:7712631
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Gene:
itsn2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX970105 [+]
ccgcgggtgccattttgtttacaagagctagcctgtgggccactgattgttacatgattgcattagatgcactgatctcccaaacacttcctcattgcaa
tcataacaacgagagaccccacgtgccacgaatgatgcctgtgcctctgtatgtagcctcagtatgagagctttcctgtgtgccactaatggcagtaaag
cctccttgtgtgccgctgagtatcaaaagctccttatggtttaagaaccttcttatttgctaataattgtgtaagagactccatatgtgccacataccga
attagagtcctcttatgcattactgatagtattatagcctccatatctgctatggttctaggctacctaggtgatagtgattgtccaacagcctcctagt
gcactgctggtggtctgagggcttcttcaagtgctactgattctaaaagaggcttgtaacataccactgatatatgtccacctccagaggcctccttaca
taccactgatatatgtccacctcgaggcctccttacataccactgatatatgtccacctcgaggcctccttacataccactgatatatgtccacctcgag
gcctccttacataccactgatatatgtccacctcgaggcctccttacataccactgatatatgtccacctccagaggcctccttacataccactgatata
tgtccaccttcgaggcctccttacataccactgatatatgtccacctcgagaggcctccttacatactactgatgtatgtccacctcgagaggcctcctt
acatactactgatgtatgtccacct
3' EST: CX970104 [+]gaaagatctaggaaggcagcttctaggaacgtatgcccctcatttgccctaattcttctgcaaacccaaaccctgataggtcaggccccagcaggatgca
ccacgtgaccaccagtgatcccatttaggccatcacttcttgactgttaccctaggtttggaggccagatacagctggttctctgatatgaagttacaag
agcagatcatctcctttctgaggcataaactatatgggataatgagcactgtggctgagggctctagcaagtccaacactacaaaatacgcaaggccaca
ggacatccttacaataagtgacagacgataccaaagctgtaagatagacatgttctcctaccagatctatgggtataaaggtttggggcagccttgtgct
cttattcacacagttatacgcttacacactcaccccaaggacacacatgggaccaggaactgtcagggaatgatcctcagaaccataatggcgccaaaca
catcatataagaatcccttgcaaccattgtgcctggcttgaatgacagtggatgagctttcttctatagagactgaaggaaaaatactgtttggagttgg
acttctgaaaatgctggttgtcaaagtgtatctattcctgcaacaacatctatcaagttctaataattgagactggtgggctgcagcaaatagcctgacg
cagaatgtgcaccggtcacttccagctctcaagctcatatctactgatcccaacatccacccaaccaatggaatgtagctacatcatcatgttagaaccc
caatataatttccagctgtccacactgccatttgttctaacaggacgtgttacactaaatgcgcaatggtagaatga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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