XB-CLONE-3557864
Clone Name: IMAGE:7712825
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Gene:
fcrl3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX970125 [+]
atttagcagaaggcttggattaaaatctcctaccacaggcgtgtcttagcaatacaagtggcacaggaaggggttaaagagcaggggttaaatagcagga
atcccagcagcaccaacccccccccccccccaccacttaccgctcctcgctctcctgcggctccgtcaccatggcaaccagacgctcctgctgtgtgcgc
atgcgccgcctacagtaacaagtcgccaagtctgggaaggagcgatgcattatgggaatcttctctacactgctcagcgtttttttttcctgtttggctt
ctgatcttctaaacaggtgaaatatatggagacttaagggcactattgagacaactgaaggtatgcctgcagcttgagattaactctttactagcctttc
cttctcctttaataaactggtgcacagcgttgtaccttggtatgggacaaaccaaccatggcacatggatgcaattctccgtatttaagtttaagaataa
cttggtggaacctcctatacagaggctgctcttacaccaaaaaagcaacccgacttatgaaagaagtagtatgggcctggaatatgggctaaattcaaaa
ttaaatacatatataaagatatattcaaggaagggaaactgctggacttccaggccctaaaatccaaatttaatttaccaaataggatgcttttctgata
tctacaactgagacactctcaattccaaggaacaactgtagaaaacttcccctctcgaatag
3' EST: CX970124 [+]ttcaatcaggacatcaaaagagaaatcctataaaacaaagcagatagttattggtgattattggtgattctcattatattattacataccctgtgcatct
gtgtttcactatttttgtgctttaatgatgctaactttaaccactaaattaattcaaacaaaaaactggtcattgcctccacttatttgttaaaacttcg
aaggtgaggggatggaaagaatttagatctgtgaggggggcagttcagcccttgttttttatttgagatattaggatgtgctatagttggggtggtgtta
aacttccatattgtgtttgtctagccatggggaccagattttccagtaatctatatgtgggcattgctcggctaacttggttttcccagaactttgtgga
tggggctgaatatgctttccattccattgttatagattttcgagcatatgcaagcaggatagagatgagagctctttcagcaattgttgaggctagatcg
gtgatttggttttgtagaattccagagggagttaccgtactaggggtactgattaaataggatatgtagactgccaccgctgtccagaatctttctattt
tgttacatgtccaaaccatgtgaatgaattctgcttgggattgttggcagcgggggcattcatctacttggaaaggagacatctttgctagtactttggg
tgtgatgtatgccctatgtaaaaaattatattatagtggcctctagggacagattagtacctgttgaaagactgattcaagcgtttccttcattgctgtt
tgtcatgtcaggtatatctatttgacatttattgtaaagttttgt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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