XB-CLONE-3557842
Clone Name: IMAGE:7712827
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Gene:
efr3a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX970141 [+]
ccctcttctaagaaaggatggttagatctgaagaccagttagacatgtactatacatccctcgtctaaggaaggatggttagatctaaagtccaaagggc
catctgctatacatccctcttctaagggaaagtggttagatctgaataccataatatgactatagtgtgcaggtctcttttccaaggactaatggtcaga
tggaaagaccataggtgtatcaggttgcagatcccagttctaagaagaagcagtcagatctaaaggctatattcatagaaaccataagtatcatctgtgg
aaacttgtatgcatttgactagaacaaatatgctatgtattgacttggctacagtatcaacaaaaccgaaccactgtgtttcattgaatgtacgtaaaac
atagatagcagaacttcaggtgatgatcagcttgatgggactaagtgaggagactgcaataatgttagtttacaagctttagtagcctatctgtgttgat
atttctgctgctgcaacatatccgattatgctggtgcagttggtcgtcttgatgtgtttgtttttctgtgcaatgctgagtgcttttacgtcttttgccc
ctacttgggagtggtgatcctaaacctctgcgggtcaccagtgcatggtttctgagttacccataatgctgttcgtgcccggtggttacgactcattttg
ttaaaagtagtggggaaccaaaccactccctctgtggcattgcatttgtgtctagccaataaatactgtgcttgataataagtactctgtctatagggta
gtaagtactccgtctatagggtagtaagtactccgtcta
3' EST: CX970140 [+]ttttattgtacattgtgcagggaacccgtacatacatggatactgccgacacagatttaacacatacaaactggcacacaaataaaacctccacgcgaaa
aagcagcaactttcagccaatattgggcaaagtattttttataggaatttaatttgtaaataaaacctattcatttaaacgaaactcttcaaagaaactt
taaaaacaaaaagtctaaacatttatatcaggttctaaatgataaaaaacaacaacaaaaaattagacacttactgccttaaaccttaaaaccaaaggat
cgaattgtcagtagcttttgtaaacaaacccctgccgttcctgtacattaattgcattgcacatatcgtttcataacccttttcaaaaatatttcagaaa
caacgtttttcagtcgcggcgaagctatagatactttattttggttttaaaatagactaaacttaagtcttttaatagcgaagaaccctactctggcgga
tttctatattttcttatacaaaatatatttttaggataagatttgaacctttctgacttacgaagacgtcgagataattgaaatctatttctttagagct
atactttcgagtgtataaaataacgaatctgacgcaggctgcgagaatataaagcgaaacgcatgaacactttttgcattgcgggatagcggacgagtgt
aaatatattgcacattaatactacggactgagtacttattaccctatagacagagcacttattacactatagacggagtacttactaccctatagacgga
gtacttactaccctatagacggagtacttactaccctatagacagagtacttatttatcagcacagtatttattggctagacacaaatgcaat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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