XB-CLONE-3557829
Clone Name: IMAGE:7712781
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Gene:
slc34a3
,
asah2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX970152 [+]
ccagtaccttggtatccctggcgcaatctggaaaccgaaatgagtttcgccgggcatttggcggttctgctgtacatggcatttttaactggatgacggt
catagtgttgctgcccgttgagcttggcactggatatctctaccacatctctaaagccatcatcaacagtttcaacatcaatgcagggggacaagccccc
gatatcttaaaagtcattacggagcccttcactcatctgattatacggctggacagttcggtcatttccggaaatgccattggagatcctgagacaaaaa
ataagagcctgatcaagcactggtgcgtgacggagtccaacacggtcacccaaaatgtctccgtgtccaacatgaccgagtgcaacatatttcagtgctt
cgaaacagagaacggaacgttccgggaaatgaacatcactataaaggaaaacattgataaatgcacgcacattttcgttaacaccaacctctctgacatg
gccgtcggcttcattctactgacggcctccctgttggtcctgtgcgcatgtctcatcctcatcgtcaaattgctaaactctgtcctgaaggggcagattg
ctcaggcaattaagaagatcatcaatgcagattttccgttcccgtttacatggctaagtggatatctggccattattgttggtgcggtaatgacctttgt
ggtgcanagtagctctgtgttcactgctgcgatcgttccattgatcggcgtgggggtcattagcatggagagagcctacccgttgttcctggggtccaac
attggtaccaccaccaccgcagtgttggcggctctagccagtcctgccgatgccctc
3' EST: CX970151 [+]ttgtcacgtgacttgaaagaaagacgatccaccaatatagacttggggaacaggaaaactgctgcggggttggtagtagccacacacttcaatatagtag
agtcctggctcagcggataaaggaatgtgccattcagcggtggcattgctctgattgccagggcccttgtgccaataatatctggtctcccaagaggcat
cattatataacatgacacgtgagttgtgttttcttacatatacatacgatgactccgacatgcgattaactccacttcgtgggtttgctgcgacaaaagt
gcatgaaacaatgtctccacgcctataccgtgggtatacgtcttttaaaacatccccaaagtctttgttcgctggagtactgtctggtggcacgggtggt
aaactgtttggaagaaggctctcattgatgagaggtggccgtgggccttttggcaattcatctgttgtgttctttgagcctgatattcttcataggtggt
gatgtagtgagtgtagacattgcacaaaccagaaaggacaacatctgtgttgagggttccagatggatcaagtatcttttttacttcttcttgtagtctt
cttcctgccattgttctgaaaaaaataccttttagtttgtgtactatgcctagagcctaatcctttcttcaactattctgcaagagtccataggtttgta
ttattttgagtcttcctattgctagggcatgtttgtaagctgccaaacatctatgtccaaacatacagctctatgtctcttttgtctgtcagtctgtttt
agagtacatctaggtatagtgtcgggatgggcgtcctagggacttctaaaaaaccaaacagtgagggcccattctcacaaatgtagacagta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: