XB-CLONE-3557808
Clone Name: IMAGE:7712735
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Gene:
mmd
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX970166 [+]
gatagacaatcccatctactcgtgaatacagccagctataaagtgctgtagaaggtaaagccaaccctgccttacaggttatagtcccacaaagcagctt
ggtgggctgcaatgcctaagtgagccagtcagtctatattagcatgcttattgatagcatggacttattgagaatgccgatgggcatgccctacagccat
ttaacaaaagcagtagtagcttgcaaggatgtgtgataactagctttaagtcacttgctgtctaacacaaggatgttgcctgtactgtacggaagattgc
tgttctaattgcagcattcagaccaatgacacgtcaacccagagactgcagtgtgactgccagctcagtataataggctgcatcataagccatcatgatg
caagctaacgagcacctagcatatttccactacttgtctgacaccagcaatggacatgggcatctcggcactttcccactgtcgagccctcgctcatgta
actgcagaatgtctctggctcggcctataggaaatcccaattttagaagttcaaatccttttcattagggctaggaagtcaaagaggaggtttcttaacc
tggaaatattaattggcacatttctttctccagtcatactgaaatactttaacaagcttcatgattttggggtacttgcaaaaataatgtatttaaattg
acttcaattattttaagtctaataaacttggaaactagagtagtaagtatcattgcttaagattattatggaaatgctgtttgttcccatacacttcaat
ccagtttgccgagttgagtactgattttcaccataactccggtgtacgcagttttttaaataagtgctccgtaactt
3' EST: CX970165 [+]cttttaacaagaactttatttttacactcaaatgtatgaggatgacagatacaaatgtatacagaaaaaatgcaaaagattcatatacaattacagttaa
tactttcagagggcaaaggacaatctacaaatgctacgataatcacaaaatacagtgagcagctacacatgtttagtggtttgcaccatattgttacaca
gtacaataatttgtatgaaatgaagtttgaaagaagtgtagaaaatataggcagggggcattctcccaggctgctgccactgtctgtggggaataacata
gtaagacttgtattttaggggatattcttttttaattgctatacactaatgctgagtaagggttctctcacattaatgttcccatttgattgacaggaaa
tgattaaggacacatctggctgtaggaatagatatgtatataagctccatattattgggagttgctgggccagcgaatcacttttattttgcaggttgca
ggccaaacgtttatatacgtgacctgttctgcccacttcagtgatgtcatagcgggggcggggcagatacaggttggaagagggcaggttaaggtagggt
gcaggtgcacccttccagccatggatatttccttatggttggccatgcatatgggttctattcagcagcagtgtgcaaaatttgcatcagccaacacaag
caacatggctgctcccacagcataaatgcatatattcagaggaggtagagaatgaatataacccttaagtataaataaaccacatggctataaaatactg
atgctgccaaaagccacaatcag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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