XB-CLONE-3557759
Clone Name: IMAGE:7712883
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Gene:
ythdf2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX970200 [+]
atggcttcttataaagagaaaaggggtacaaaatgactttaggtggacatatcctttctaaagttcacattgaacagttgggttaccaaccctgcagatg
ccaaaactggcaataacaccttcgtctgacttaaagttaatggaataaacctattttaaataaggttctttcttttttccaaacattttttttttaaact
gtataccatccaaaacaaaagttcccagattaaattaaactttctctagttgaatttgcccagtcaagtctgtgggaataacaaaatgaaaatacttaca
tataaatttagatatgcagagacagcagattttgttgatgcatctggcacacaaaacatttttttagaggaacaggtgtggccctatgttgcagctttta
taatgtaagctttatctgtatatgtgtataaacacgcacacatacatgtggagcactgaaaaaataaagaaggcttttgccacaaactaaaaagaaataa
aggaaaaagagggccttaggatataaaggaaaatacatctccagatgctaggaatgagcatactatattttaatctgatttgtaatctgggttaaaaccc
aaatttaattgaattccagtattcaatccttgttgtgagttctggatttgatttaatttgaatttaattgctcaagtaaggggactgttaccctctccca
ttgtccttcaataaggtaactgcaggtcctatttgccaaagtccatcttgtcaccgctgaacatctgttaaaggggacataatttggt
3' EST: CX970199 [+]tcgcttcaccaaaaggatggcttggatgatgatgaatttgagccatatttgcatacacaagagcggcagaacaatgcatacacagcactgtcagactcat
acttgccaagttattacagcccatctatcagtttctcatactccctgggagagggggcctggtctactgggggtgaccctccacctactatgccatacct
aacttcctatggccagctgagcaacggagaaccacattttttgccagatgctgtgtttggacctctggggagtgccccattccttggacagcctgggttt
aacttttttccaagtggtgtagacttctcagcttggggcagtggaggcaattcaccaggagcctctgctcctagttctggatatgggggaagctatgcat
atgcccccagcactttagggggagctgtgttagaggggcaagcaagctttggtgggggaaaggcagtgggaagcctagaacagggaatgggggctctgaa
actgagtggagaagggggtggtctaaaggctgctataggtagtctgccacctgcatctgtggcaccacccaaacaggcatcttgggcggacattgcaagc
aaacctgctaagcaaacccttgctaaaattcttcctccccctccaccctccaaacacaatatggagattggaacttgggataacaaagtatccacagtca
gcgtttgccaggcatcatctccaccgatccttcagcagtctatccccaatgctgcaatagctcctcatgctcctacacgttggtcagctcatcgtaacag
aagctcg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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