XB-CLONE-3557644
Clone Name: IMAGE:7712607
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Gene:
upf2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX970278 [+]
ccgaggcttttattcagcagctgccaaactgtgttaatcgtgatctaatagataaggctgcaatggatttttgcatgaacatgaatacaaaagctaatag
aaggaaattggtcagagcactattcattgttcccagacaaagactggatcttcttccattctatgccagattagtggccaccttgcatccttgcatgtct
gatgttgcagaagacctttgttccatgttaaagggagactttcgtttccacgttagaaagaaggatcaaatcaacattgaaacaaagaacaagactgtga
gatttattggagaacttgccaaatttaaaatgttcagcaaaacagatacgctgcattgtttaaagatgctgttgtcagacttttcgcatcaccacataga
aatggcatgcactctactggaaacttgtggcagatttctttttcgatctccagattctcacttgcgcaccagtgtcctgctggagcaaatgatgcgcaag
aagcaagcaatgcacttagatgcacgctatgttacaatggttgaaaatgcctactattactgtaacccacctccagctgaaaaaactgtgaaaaagaagc
gacctccactacaagaatacatcagaaagcttctttataaagacctttccaaggtcaccactgaaaaggttttgaggcagatgaggaagctgtcttggca
ggatcctgaagtgaaggactatgtaatttgctgcatgatcaacatctggaatgttaaatacaacagcattcattgtgtagccaatcttttggcaggactg
gtactttaccaagaggatgttggaatacatgttgtagatggagtgtt
3' EST: CX970277 [+]atagggcgagaggcgaaaaattgggaaaaataaatctgttacttatcaaattaaacttgtaaataccaaagagctagcctttaaaggtaacatcactaac
tgcttcactccttactctagtacttagctgtaatcctgcaacaggctaaatgttgagaagccaaaatagagcatacgttggtagtctggccagttcttgt
gttcagctggtataggaatggaagagaaatgcaaactgcacattttagctctatacaaattttggcaaattctatgcacagccaaagaagcccagtctgc
tctggcatttgccaaaaggcacagtgtctttccattttacactgtactattacagagaaaaagcaaacacattttaaacaaaaaatcttcttaggagtat
ttgttgcactccagatttgtctgacttcctgtgttcaacgtttccagctttttgcccctttgtgccatattggctattgttttgcaactctgtgttcttt
caaaaagtgtgacgtccagtcctacatcttttcgctggatccttctaacagtgcaaaactacaatacagttgcaatgcatgtccaccttaaaggaaactt
ctgtctacttttagccaaactgcctgctaataaagtaaaccctcttaatgctttccatccaatgtaaggctgtcttgagcgtttttgcagatacaaaaag
ggccccactgtttgtgtgtcagtggtgcctacgcttgaagcttctttctgtctgaagcagaagctggtattgctgtcttcgccttgctcccaagccctgt
cacaggattccatcccaaccttctgttctgaacatgctgcgatgtgctgctgatcttttacantttctgggaggttacactagccatgtt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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