XB-CLONE-3557635
Clone Name: IMAGE:7712751
|
Gene:
phf20
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX970284 [+]
tgtatgtgtcttgcattattgtagggaaagagtctaggcattgggcctagtaccgcagaatacccactgagtggttgggttttgggctaagaaggcacgt
gtgcaagctgggggttggggggagtcaagtcacaagtaatcatttgaaaagaaaaatctgatttatttattcaccacctccttcatttgccccttagcta
tgaaacctcttgggtttcaggtctggttgatgggccagtcaacccctagatggttaaaggggggggagggacacatatacagtgtataaagaaagctata
ttggaagtaatgtaccccctactgcaaaatttagggtattaatggtcactgaggcgttttatttgttaactggaaaccttggtactcgttatccaagcat
tattgtttgttggccacggtctaatacttctctttagagaagttgtcatttgtttttaaaattctggataatccgcttgaaaagcaaatagtagagactt
gagttgttgcaaccttgtgctatatttcctgcacccactgcaggattatggctggggaagcaatcgttattgacgtattagtcaggctggcacaatctgt
tctttaaaaaaaaataaaacaaaggtacaacaaaggtcgtttgtcataaaagttggttttacgtccaatagcttgtagaggtttctcccaaaatattatt
cccgatttcctgcacattagatgccagtctcaaccctggtttgagttaataatatatgtgttgtgctcctgtcctgntgcaccgatcagctggtggtgtt
tgcctacatggtccatttgtgcccctctctagacacgcctctt
3' EST: CX970283 [+]ttaacgctctctgactgtttgtcagatttgctgtgtgttttctggatctgattctccttgctcttctctttgagggaaggccggtgagagtttgacgtat
atgaggcatgcattgaatgtcgtctcttttctcctaacctctccgctttgccatcctgaggggagctccgcaatgatatctgaagaggcgtgtctagaga
ggggcacaaatggaccatgtaggcaaacaccaccagctgatcggtgcaacaggacaggagcacaacacatatattattaactcaaaccagggttgagact
ggcatctaatgtgcaggaaatcgggaataatattttgggagaaacctctacaagctattggacgtaaaaccaacttttatgacaaacgacctttgttgta
cctttgttttatttttttttaaagaacagattgtgccagcctgactaatacgtcaataacgattgcttccccagccataatcctgcagtgggtgcaggaa
atatagcacaaggttgcaacaactcaagtctctactatttgcttttcaagcggattatccagaattttaaaaacaaatgacaacttctctaaagagaagt
attagaccgtggccaacaaacaataatgcttggataacgagtaccaaggtttccagttaacaaataaaacgcctcagtgaccattaataccctaaatttt
gcagtagggggtacattacttccaatatagctttctttatacactgtatatgtgtccctcccccccctttaaccatctaggggttgactggcccatcaac
cagacctgaaacccaagaggtttcatagctaangggcaaatgaaggaggtggtgaataaataaatcagatttttcttttcaaatgatacttgtgacttga
ctccccccacccccagc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: