XB-CLONE-3557601
Clone Name: IMAGE:7712563
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Gene:
zan
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX970308 [+]
ccaagtgtcacattacaggcgatccacaccataatactttcgatcgcctatcacatcacttccaagggaaagatagttatatcctgactcagacatcttc
tgccttgcctgatttattggaaccatttacaattaaaggcaaaaatgagccaatgaacaaatacagtacatttacactaataagggagatgcgcattgag
gtttataatcacaccataatattttcacaaaagaagaagcttgtgttggatggtgtaaagacagtgcccccaatcaatctaaaggaaggaatatacatat
atcagagaccattaagaatctaccttgagactgattttgggctttctgttagctacgatggcaaccagaatgcagagataactgtacccaatacacatag
aaatattctagaaggtctttgtggaaattacgatgggcgaagttctaatgattttagaaggccagatggatcgcaagtgagagatgtggtgtcttttggt
gaaagctggaaagttaagtcatggaaatcagattccagaattaggagagacatcggattaagaaatgaagaggatattgtggacttagacacaggggaca
ttttagcttgctcagcctcagaacttgcttatttgaacagcacatcattctgtggaatacttagagatattaatggaccattccaaacctgtcatgaata
tgttgatccagaagtctacatcacgaactgcctttttgatacatgtgctgaatttggaaatattgaattgttctgctctaaacttgagcaatattcactt
gcctg
3' EST: CX970307 [+]aaaatataataacagtgatagaatcaaggcatttaggcaaacgagtctctgctgcaaaaatgctgcagtaaaatcagtagaaagacaattacattcaagt
gcattggtttttgcctagacatacaaaattataatatcattctttgttttatttttttatgattcattctgaagttacatcaagttctgctagattaaaa
ttgtgtttgtccatgtgtgcctgtggttttttctgacttcattgcatttacattgtccgctgagccctggttacttgttcctttagcagatgttctgacg
tttgtgtcgttagccacattcacggccccagactggggcacagtactgtcttcctcaaatgctaggttaattaaatctggctcattatatatagattcac
ctccacgattcactgatattctcgacaagctcacactcccatcttcagagctatttgattcgacaaggcgttttttaccatcccggtttttgatgaaaca
aaaggcagcaaaaacaaaaataaagccgataaccaagactccaaccacaactccgattacaatgtaaataactctgttgtcatatgttttctcttcaatt
tcacagaagaatccagaataagtttcagggcacatgcagtacaaatcaccagtagtattcattccaagagatccttcaacacatgtgccaccattttcac
aagggttttcaaggcagggcccaggtatatagcatccacggatctcatatgctgttgtgcagatctcattaggtttacattggatttcgaagcaaactac
agatgaagtgtctgtgcacgtgcagttctgggtacaatcatcagtgatgaagac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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