XB-CLONE-3557591
Clone Name: IMAGE:7712803
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Gene:
setd3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX970316
[+]
cgcagtcggtgtgtttttcgtgattctccgtggcagtttttgactactgtaatttccagggcgacgaggggaggggaggagggagctgtactccacaatg
ggtaagaagagcagagtgaaaacccagaagtcggggagtggagcagctgctgctgtgtcaccgaaggaaatgcttaatttgattagtgagttattgcaaa
agtgcagcaacccaaattctactcccgggagagaatgggaagagtatgtgcagatccgtgggcttgttgaaaaaatccgcaaaaaacagagaggtttatc
tgttgtctttgatgggaaaagagaagactattttcctgaactaatggagtggtgcaaagaaaatggagcatctactgacggctttgaattagtggagttt
ccagaagagggcttcggtttaaaagcaactcgagaaataaaggcagaagaattattcctgtgggttccaaggaagctactaatgactgttgaatctgcaa
aaggttctgttttagggcctctttactcacaagaccggattttacaagccatggggaatatcactctggctttccatctgctgtgtgaaagagcagaccc
taattctttctggttaccatacatcaaaacgctacctaatgagtatgacacacccctgtattttaatgaggatgaagtccagtatcttcagtcaacacaa
gccattcttgatgtttttagccagtacaagaatacagctcgacagtatgcctatttctacaaagtcattcagacccatcctaatgccaacaaactgcctt
tgaagattctttcactttcgatgattacaggtgggcggtttcctctgtcatgancagacag
3' EST: CX970315
[+]gactgtgcaatacctttagatttggaagagatgctgcagtggtctgctagtccctcctggactgaattggctaaagcttttatgtggacccgtatgcctc
cgaactcccagtgcctgaaagtgcctaccaatacttttaaaaaatggatatgtcaaatttgcttatatacataaacacctgttctactgcaaaatatacc
tttatttcttaagaccaggggaaaataaaaaataatgcacattatttcagtgccttggataaactaaaataagcatgctcagtccttttgccaaactttg
caggaggtgccagtcaaataaatacttaaaatgcttgcctatcatatgtgcaggtttttgcctgatgtaaaccttttagagctggagatctcttcaaggc
aaggattttacaatacccttaacaaagagattcaaactttatgaataccaataacattaaaaaatgcaggccctactacctatagatattatttcaacct
gaagacatactggccatgaacctctttttccaattaggctgcttaaattacacgctagatcagtttcactggtgagagtttttcacacattggaggaaaa
aaaataaatgtacacaagtctaaagatcactctaggttgttcactccttaaagcaggacaagagatttaatagagctccatctgtgtgtgggtgtttctt
ctgaaaagaaaccctacactgagggtgaaaaaacaaatatgaaagcagcaatgcttatctgcagaaacaggaactcacaccaatgcaaagcagtgtttca
caaactttcgccggttttaatttgaataaacagtcaatctttttcacaactgtacttttgcctttttgatggtttat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: