XB-CLONE-3557585
Clone Name: IMAGE:7712899
|
Gene:
csde1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX970320 [+]
attttttttgccattaaacagaaaaactggagccaagtatcattttataaaggtaatgtgagtctccaaaaaaaaaaaaaaacaaaaaaaaaaaaatact
ttccctctttccgtagaaagtggcttgatcttttttgcttaaaataaaaagggcaacttaatttaatattttatgcgagcgggtaatggaccgaaacaaa
taaaatagggctggggggagggtagtttaaaaaaaaaaattatatattaggtaccagctttctaaatttatcttgatgcgaatgaccaatataactatca
aaagactaaatcttatgataaatcggtgctgtaaacaaaaacaaaaaaaaaaacaatgtttcaggcacatttttcttccttttaaatccattaatgtttc
aaaagctgcagtgtcaaagcagcaaaaacaaaaaaacaaaacaaccgtgcattttcgtattaatttaacacacatggcacatttcaggcttgttgtactc
tgcacttccaaagtcacttgttaacacaaaaaccaaaataaattttgaagaaatgcattcaaaaactaacagcgtgtgaatgcagagttaattaatcccc
tctgaaaatgtgaatgtttggctgtcactggaattagaaaatgcttcattttttatcattgcttagggaacactgcataggatatattccaccatatcta
tgtggtcctttgatagaaaaataaagcatagaccaatgctgaataaaaaggaattgctatgttaaatctaaggcaaagtaaaaaaaaaaaaatgaaaaaa
aa
3' EST: CX970319 [+]gctgcttcaagttcagatcagtcaaggaatacctgccacaacagtaactaacaagaccaaagaagagaaactgcgctgaagacacaacacttgatctgaa
acaagaagtttgtgcctactcaacagcttcacaaaagcacttcccaacgctgctattagtctttgttttatcctttacgctgcattttgcaactgctgag
tgcagcgtaagtcatccctcttagcttgatagatcgttttctcttgctatctctcttttaatactagcatctaacagccttgaaggatttactgacggag
aattggaaaatactactgaacgtgcaatatctgaatatctcggaaagatgagctttgacccaaaccttctccacaacaatggacacaatgggtaccctaa
tggtactgcagcactgcgtgaaacgggtgtcattgaaaaactgctgacatcctatggatttattcagtgttcagagcgccaggctagacttttcttccac
tgctcacagtacaatggaaacttacaggaacttaaagtaggagatgacgtagaatttgaagtttccgcggacaggcgcactggtaaaccaattgctgtga
aactcgtaaaaataaaaactgaaatattgccagaggagagaataaatggccaggttgtgtgtgctgtgcctcataacttagaaaccaagtctccagctgc
tccaggccagagtccatctggaagtgtgtgttatgaacgaaatgnggaggtgttttatttgacatacacaccagaagatgtagaagggagtgtccatcta
gaaactggagataaaataagctttattattgaaactaataagcatactggtgcagttagcgcacg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: