XB-CLONE-3557463
Clone Name: IMAGE:7712768
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Gene:
eif3b
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX970401 [+]
ctttattctcggggagaaacgaattccaaccacggagagacgcaagaatccaacgtaggtagaacagagacaaacaggaaagtgccgaggaatgctggag
agcgaacgacccgagagagacatggaggaggaagagtccaccgaggaggaggaggaagaagggatgtcctttagcgatccggagggattcgaggacgata
tcagtgaagaggagttgcttggtgatatcctgaaagaccgtcctcaagaggcagatggaattgactctgtagttgtggtagataatgtgccacaggttgg
acctgaccgcttggaaaaactcaaaaatgtcatcaataaaattttctccaaatttggtaaaattaccaatgaatattatccagaagcagaaggcaccacc
aggggatacatttttctggagtatgctttgccaactcaggcacaagatgcagtaaagaacgcagattgctacaaactggacaagcaacacacattccgcg
tgaatctcttcaccgactttgacaagtacatggttattggagatgagtgggaagcacctgaaaaacaaccatttaaagattttgggaatcttcgctcttg
gcttgaggatcctgactgccgagatcagttcagtgttatatatgaatcgggtgatcgtacctctatcttctggactgatgttaaggagccggtcccagtt
gaggagagagcaagatggactgagacatatgtacgctggtctccaaaaggcacttaccttgccacctttcatcagagaggtattgcactatggggtggag
aaaattcaagcaaattcagagattcagccaccagg
3' EST: CX970400 [+]tttttttttttttaatagatgtttactttaaattccatcttaatcccacattgttgaagcctgaatggaggcggataaagtccactgtaatgctggttca
gtgcaccaaatgcactgattgccgctgcttcattgctattcatctgcaaaacagaactgaggattgtccatttgattggtttaaatgaacagcccttcac
aagatgatccagcaggcacagagatgctgaaaaataattcatggtgcagtgaactgtagcccagaagataccagcagcacagaaagcacaattttatata
gccagagatggagcaagtggcaggtccaagaagtagtactatcaatttccaattaaaaagaagtgccctttaaaatatcctccaatgagggctaaaggca
caacttcagagagatttatatgtccattcaaggttgccgaggagagataaaatggaatcttggagtctgaccccctggcagagaacagtcgccaaatatg
ttggtacagatcgttagcagactactcttccactgggataatttcttcagttacaaagaactctatagtctcttcctcccagtcatccacattgctgtcc
agctcatctgtgtccacgcctcctctgatctccagacgggcagctttctgttccatgtatagttttgctgccatctcccggtatgtcttgtattcttcca
tcatggctcgtctgcgctctattagttcctttgaggctttagattggcttaaacgatctttttgttcaaatatctttgaatacttcttcaaatccttctt
gatttgctttatttggtcttgg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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