XB-CLONE-3557443
Clone Name: IMAGE:7712818
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Gene:
eef1a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX970414 [+]
gcaccagcagaaggagccaggcatgagtcagtcccaaaatgacctcgtctacctggaaacaccacgatctggggaacaccccaagaagggggcgactctc
tcccgactcctgcacaggaaacgcctcaagaacaaagacaagagcccgacaccgggcactccgacggggaactgaaaggcctttcatttcaaggcttcat
attagcattactatctgtgcatggatgccttctgcactccaacggaatcctcggaatcccagtggaataaacagaacctgtgaatattcctttagctcaa
cactgtgaggtttttttgaagcccagaaaaagccataagaaccttctccttcaccctgacgggcaggggaggaaaacagcattgcttgcaactaagccag
cctcctgatggagggcgaggagatacgccttgggaaagggcctccgtctcttggtagcaatatatttatgcttgtgctgaattcaccgttagaagtggcc
ggctcgggccgcgagtctccggagagccatttcctatagagaacacagcgtggtgtcatttactgtagttttcctatgcatgtaatgagtggagtccagg
gcagcagccaatcccgccgacgggtttagaaagctggattctaattggctaatatctgtgcaaggtttgctgcacttttgctttcgtagtactacatagt
agtagaggtgtgtattttttttataatttatcacgtanaggcccctcttgtatatgtatataggaatacagctgtatataggctatgcaagtatcgtggc
aactgtaaggtatatgtataggtctatgtgtatacaattacagaacataaaccag
3' EST: CX970413 [+]tatagggcgagagggggaaaggaaaagactcacatcaacatcgtcgtcattggacacgtagattctggaaagtccacaacaactggacatcttatctaca
aatgtggtggtatcgacaagagaaccatcgaaaagttcgagaaggaagctgctgagatgggcaagggctccttcaagtacgcctgggtcttggacaaact
gaaggccgagcgtgaacgtggtatcaccattgacatctccctgtggaaatttgagaccagcaaatattatgttactatcattgatgctccaggacacaga
gacttcatcaagaacatgatcactggtacctctcaggctgactgtgctgtcctgattgttgctgctggtgttggtgaatttgaagctggtatctcaaaga
acggacaaactcgtgagcatgccctccttgcctacactctgggagtaaagcaactgatcgttggtgttaacaaaatggattcaactgaacccccatacag
ccagaaaagatatgaggaaatcgttaaggaagtcagcacatacatcaagaagattggttacaaccctgatactgttgcctttgtacctatttctggatgg
aacggtgacaacatgcttgagcccagcgccaatatgccttggtttaaggggtggaaaatctcacgtaaagagggatctggcagcggaactaccctgctgg
aagctcttgactgcattttgccaccaagtcgcccaactgataagcctctgcgtctgcctctgcaggatgtctacaaaattggcggtattggtactgtacc
agttggtcgtgtggagactggtgtccttaagcccagcatggtggtacttttgccccctgtaatgtaacaactgaagtaaaaatctgtgaaatgcaccatg
aagccctt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: