XB-CLONE-3557262
Clone Name: IMAGE:7712692
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Gene:
eef1a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX970539 [+]
cccaactgataagcctctgcgtctgcctctgcaggatgtctacaaaattggcggtattggtactgtaccagttggtcgtgtggagactggtgtccttaag
ccaggcatggtggttacttttgcccctgttaatgtaacaactgaagttaaatctgttgaaatgcaccatgaagcccttagcgaggccatgcccggtgaca
atgttggctttaacgtgaaaaacgtttctgtgaaggacgtccgtcgtggcaacgttgctggtgacagcaagaatgacccaccaatggaagctggtacctt
tacagcacaggttatcatcctgaaccacccaggccagattggtgctggatatgcccctgtgttggattgccacacagctcacattgcttgcaagtttgct
gagctgaaggaaaagattgatcgccgttctggtaagaaactggaagacaatcccaagttcctgaagtctggtgatgctgccattgttgacatgatcccag
gaaagcctatgtgcgtggagagcttctctgactacccccctcttggtcgttttgctgtccgtgacatgaggcagactgttgctgtaggagtcatcaaggc
ggtcgataagaaggctgctggagctggcaaagtcacaaagtctgctcagaaagcacagaaaggcaaatgaatatttccagcatctctcacctcagtcata
accagtggtggaggattggtctcagaactctttctatcaattggccatcagagtttaatagtcaaagactggttaatgattacaatgcatcgcaaaagct
tc
3' EST: CX970538 [+]cttttacttttttttaatggtctcaatttggtgacagattttggtcaagttgcttccatttagaaattactggatttaaaacactaataacttaaaaact
gccacaaacaaatgtgtccacgagcatttttttccttctgaagcttttgcgatgcattgtaatcattaaccagtctttgactattaaactctgatggcca
attgatagaaagagttctgagaccaatcctccaccactggttatgactgaggtgagagatgctggaaatattcatttgcctttctgtgctttctgagcag
actttgtgactttgccagctccagcagccttcttatcgaccgccttgatgactcctacagcaacagtctgcctcatgtcacggacagcaaaacgaccaag
aggggggtagtcagagaagctctccacgcacataggctttcctgggatcatgtcaacaatggcagcatcaccagacttcaggaacttgggattgtcttcc
agtttcttaccagaacggcgatcaatcttttccttcagctcagcaaacttgcaagcaatgtgagctgtgtggcaatccaacacaggggcatatccagcac
caatctggcctgggtggttcaggatgataacctgtgctgtaaaggtaccagcttccattggtgggtcattcttgctgtcaccagcaacgttgccacgacg
gacgtccttcacagaaacgtttttcacgttaaagccaacattgtcaccgggcatggcctcgctaagggcttcatggtgcatttcaacagatttaacttca
gttgttacattaacagggggcaaaagtaaccaccatgcctggc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: