XB-CLONE-3556867
Clone Name: IMAGE:7713065
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Gene:
eps8l2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX970811 [+]
cagaaatcctattttggtgtctggtagcacctatgagaatgcccggtgtgtaaagaccttgattacctgaactcattggctacttacactctgctgatgg
ccgtcgtttgaaatacagcagcaagggaaattagtgcgaagaaaattgattattccgtaaacttagtatcatgtcttattttgcagaacaaaggaagaaa
tattctaactccaatgtcatcatgcaagaaacatcacagtacaacgtggagcatctggccacgtttgtaatggataagagtgactctcttggcacagtag
acgatgccatcaaaaggttgtttcagttggagaccaaagacaaaatctgggcccaagaaatgattctgcaggtcacaaacaacaatatcaggctgctgga
cagtgtgacacaggaagagctggagaactttcctttgcctattgtccagcactgccagaacttgtcaggccctcaccgctacccacacgtcgtattactg
gtgtgccaggatgcagaccagcccaagcccgacatacacttcttcaactgtgaaatggaggctgaggttattcatgctgacatagagagcgctctcgcag
atataaagcaaagcaagaagatacgaccacagacactcaagattaaccaggagaaaatcaagcaaagggattccattctgcctcctgctactgcccccaa
acctcccagagacaacagaaacagggtgatgaacccacctcagcccaatgacattgaacacgaccgaagaagcaacgtctcccaggaacccgaggagccc
cgagaggttttag
3' EST: CX970810 [+]aaaaatttcaaatcttggttattattaaaacttgaatcacagaaaacaaatacatacaaatgttgataaatagccccattgagtagtcaggtttctgtgt
ttaaaagctacagcactggagacactgctgtgtaatgagccccaacagcagccctagagcagaggaacatgattggtggaagtgaccccgttataatcct
gatcagtttagttccattgatatttagatagtagttttgcttcaatattgatagttctctcagatgtgtatacatgtgtttctcaatattgatagtttta
tttttaccttttttatttcaaatgcaaatattgtatttaaaatttaattcaatatggttttgttttttcaccactcccacttccatgcaactctaaacat
acacatacagggaatgacagtatcaatatattagcaacagtataattacaatctgcggcttaagacaaaaacaaatttttctcataaaggtaacttgatc
atattgctctgtgttatggaaaacatatttaatgattgcgaacataagggcacatctgacattcaggatccaacactgttacatagcaacacatggtact
taggaaaagcacatgataggaaggaattaaagcagacagagtaaggcctcttttggtattgtgttgacaatcttcaaagtcttctaaaatattgtgttta
gtatttcaatcattttaatctgagcgcgtgtacagtctggatgatattcgtccttacaaattgtcactgtacaactctgcgatattcacatactgaacaa
cctgccaactgtgga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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