XB-CLONE-3556845
Clone Name: IMAGE:7713067
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Gene:
actg1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX970827 [+]
cttaaggaaacctccagcaccaagaaataaaatggatgaggaaattgcagcactcgtcgttgacaatggctctggaatgtgcaaagccggctttgctgga
gatgatgctccccgtgctgtgttcccctctattgttggacgcccaagacaccagggtgtaatggttggtatgggccagaaggacagctatgtaggtgatg
aagcccaaagcaagaggggtatccttacccttaagtatccaattgaacacggaattgttacaaactgggatgatatggagaagatttggcatcacacctt
ctacaatgagcttcgtgtagcccctgaagagcacccagtcctgcttacagaagctcccctgaaccccaaagccaacagagaaaagatgacacagatcatg
tttgagactttcaacaccccagctatgtatgtcgccattcaagctgtgctgtctctgtatgcctctggtcgtaccactggtatcgtgatggactctggag
atggtgtcacccacacagtacccatttatgaaggttatgccttgccccatgctatcctgcgtctggacttggctggtcgtgacctgacagactacctcat
gaaaatcctgaccgaaagaggctacagcttcaccaccacagctgagagagaaattgtgcgtgacatcaaggagaaactctgctatgttgctcttgacttt
gaacaagagatggcaactgctgcatcctcctcatccttggaaaagagctacgaactgcctgatggacaagtaattacaattggaaatgagaggtttagat
gcccagaggcactctttcagccttctttcctggtatggaatcatgtggcatccatgaaactacattcaactcaatcatgaagt
3' EST: CX970826 [+]atttactgcagcgctttattttatccttacagaacggtgttttggaagttacaattctcatctggatgacatacaaattttggttttctacgttgtgaga
acattaaggcaacatacaaaaaaatttacataaattaacttggatgcataaatttacaggtgttaactgcaacatttgtaactttcttcagacccatttt
gaatgttgggaatacaacctgggttaagaaaaagactggttcttgggcctgagtgtcaatttccaaccttactataaactggaaagatagaagagattcc
ttcatgaactgaccagcctccgacatgaaacaatggagtattaagcgcttaactgtgtgtggaagtctaaacctcagataccctgtacacatctccaaaa
ctcgacttctccataaacagcccctgtaacctagaggagaagtgtcattcgcaatactgtcgcagatcggcagcctttcacagaaatgcttctttctacg
taaagcttagcttaagtgtcacccagactgtatgccaaccaggcaatataagagatgtgtacagggtacaatatgctagcattgatgccacaggaatgat
ttgaagagacaagcacaagtcttgcattacaaaatcccccaacagtgactatgtgtgtctccaattcctaggataagttcatttggaagttggccctttt
tccagtttcaagaggctagcatgaggcgtacacatttgccaaggcaaattaatactttgaagaactcacgcagcaaatgcaacgcatctgctagcagtcc
att
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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