XB-CLONE-3556761
Clone Name: IMAGE:7713075
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Gene:
washc3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX970885 [+]
cactaaggtcccacctattcagcagaaaagaacagtggcatttttgaaccagtttgttgtgcactctgttcagttccttaaccggtttgctacagtttgt
gaagagaaactttctgccttgtccctacgtattcagcagattgaaacaacccttaatattttggaagcaaagctgtcctccatccctggccttgaagatg
tcaaagttgaaacgcaacatgtccctcagagtaacatatctaatggtcatctgccaagtcagcctgatgcacagtcagttatagtatctcctcagtctga
taacaattccataagtgatggtattcttcaaaaagaggaggcaaaatcggaaaacattacaactgtttcgaaagatcctagatatgcccgatatctaaag
atggtccaagtgggtgtcccagtgatggctatcaagaataaaatgatttcagaagggctaaatccagaccttttagaaactcctgatgcaccagtgccag
atggagaagctgaagcagaagaaagttccgacagtgagtcttccttcagtgactgagagcatttagactcctcctaatccgggcagatttgttcccactt
attgcaggcatctgatctgcctgttatctcattactgtcagtttacatagatacacgttgtagctacaagtgtagtattttaggacaaaatgtgccttag
tgttaaacctgttgatgcctcagagctgtaaggtatataaaataaccttgtgtggtatcccaaaacttaatacgtttatttgatttttttttcctttgt
3' EST: CX970884 [+]ttgaggatcttgtcttttttaatggtaaatgaaggaaaacaaaatcatgtattctggacagtgattttcagcctatcatgcatcagtatctacatgcaac
tctttttatttcactgcacagaataccctaattagcttaaatcagagatgtgaagatgaatacacgtcgaggatgccagatgctgcacacatatggctta
aaaacaagctactgttatagcctatacctgaatggacggatgcgctcaccctaaaaggctataaggcaaaggtgaaacaaaaatattgttgctgagcagg
aaacttgagtttataagtatattgctttctacagaaacctttgaaaaactgtctttaaatatacagaaggttggcatcaaagcagatactaagctgggca
atgtgactgaccaaataatacaccattcaaaaatttagacaaatattattttccatatttattaaatggaaatggagtaattcaaaggctacaactcatt
taagcaccatggctgttgtatggatggcaatgctgttgtatggatggcaacagaccccgacaggccaaacaaagaggggatatatactttacttaaagat
ggatcgaagagcaattttggaaaattgataaagtggaaagcagaatgggtactggcaagtgtgtgtgcatgctcacagcatgngcctgttttatttccct
aagtacagtaatacctacttgtgcattaaatgtgaataataaacatacaaacaggataatcctgatagttttacatatacacatatatatatatatatat
atatatatatatatatatatatatatatatatatatac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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