XB-CLONE-3556752
Clone Name: IMAGE:7713219
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Gene:
elovl1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX970891 [+]
caacactggcttgctgtatattcaccctagtctttttagccatcagatattcagtacttttaactcaaagtaagaaatgaacataagttattgataattt
acaagcagtaatggtaacaaaaagtgctcaagggaagatgttgagctgaactgttttatgtcttatggttggcgcaaatgcacacacagatggtcttaca
aactttcataccatttctagtactgtactattgcttgcgcattcaagtctggatgtaccactcactttgtactgcctttgtacatatagaactggacaag
catagtgcagtgtatggaaatacacattgcacagagaacatgaaatggtaaaatgctcagacagctggatccaatccaagtaataaacaaagcacttaaa
tcacttaaaaaaaaaaaaaaaaaacagtgttaaacagttatgtgttttaccacattaggcatttggtctgttttgaacatagagactactgctgcactga
tatctttgccaaacgtttggataagatagacttggtgtttaccataaccattggtagctgttagagttgatatatccaatgtgcaataagcgtttattag
tattacacaaaaaaattataaataggttctggcagacttgcaggtagttacatgaagtccttatatctctgtgccttacccactgtataccactccttcc
ttcctggcagtcatacaatatcctattatgacatttttgatcaagcaacagtgtcaagaatctatttcaatt
3' EST: CX970890 [+]ggaagccgtactctcagaatgggtgcaaaaataccacaactttatgaaaggagcagattccaggatatatgattatccattgatgcagtcaccattcctt
cctggtgctattctcctgagttatgtctactttgttctttctcttggcccacgaataatggcaaatcggaagccttttgacctaaagccactaatggttg
tttataattttagccttgtggcactttcagcttatattgtttatgagttcctcatgtcaggctggttaactggttatacatggcgttgtgacccagtgga
tgtgtctgatactccaatggctttaaggatggtccgagtagcttggctgttcctgttctctaagtttattgaactacttgatactgttttctttgttgta
agaaagaagaacagccagatcacctttctgcacatctttcatcactctgtcctgccttggagctggtggtggggtgtcaagtttggcccaggtggtatgg
gatctttccacgctatgattaattccttggttcatgtcatcatgtacttttactatggactgtcagctgctggtccacgctttcagaaatacctgtggtg
gaagaagcatatgacagccatccagctgatccagtttgtgctggtttccattcacatttcccagtattacttcatgccaagctgcgattaccaatacccc
atcttcattcacctcatctggatctatggcacagttttcttcatct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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