XB-CLONE-3556704
Clone Name: IMAGE:7712937
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Gene:
cela1.6
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC156019 [+]
ACATTGTTTCAATGATGTGCGCTACATTGAAGATAATCAGCGAGTGATTGGAGGAAGTGAGGTAGCCAGGAATTCTTGGCCATGGCAGATTTCCCTCCAG
TACCTGTCTGGTGGCAGTTGGTACCATACCTGTGGGGGCTCCCTGATCCGTGCCAACAGGGTCCTGACAGCTGCTCACTGCGTAGACACGGTCACTTCCT
ATCGTGTGGTGGTGGGAGATCACAACATTTACCAGAATGATGGCACAGAACAGTACATCTCAGTATCAAGGATTGTGCAACATGCCAACTGGAACAGAAA
CAATGTTGCTGCTGGGTATGATATTGCAGTGCTTCATCTGGCCTCCAGTGCTGCCCTCAACAGCTATGTTCAATTGGCCAACCTGCCCGCTGATGGTGCG
GTCCTGGCCCACAACTATGCCTGCATTATTACCGGATGGGGCAAAACCAGCAACAATGGCTCTGTTGCCTCCGTACTCCAGCAGGCCCCTCTGCCCGTGG
TAGCCCATGCAACCTGCTCCACTGGCTCCTATTGGGGAAGTACAGTCAAGTCCACCATGGTGTGTGCTGGTGGTGATGGAGTCCGTTCAGGATGCCAGGG
TGATTCTGGTGGCCCTCTTAACTGCCCTGTCAACGGTGTGTACCAAGTCCATGGTGTCACCAGCTTTGTTGCTGCTGCTGGTTGCAATACATACCTGAAA
CCAACAGTGTTCACCAGAGTTTCAGCTTACATCGGATGGATCAATGCTAATATTTAAAATGTGAACGTAAAAGAAGTTTGAAGAGTACCAGATAACTCTA
ATAAAGGATATGTAAACATTGAATAA
5' EST: CX970924 [+]aattgtttcatgatgtgcgctacattgaagataatcagcgagtgattggaggaagtgaggtagccaggaattcttggccatggcagatttccctccagta
cctgtctggtggcagttggtaccatacctgtgggggctccctgatccgtgccaacagggtcctgacagctgctcactgcgtagacacggtcacttcctat
cgtgtggtggtgggagatcacaacatttaccagaatgatggcacagaacagtacatctcagtatcaaggattgtgcaacatgccaactggaacagaaaca
atgttgctgctgggtatgatattgcagtgcttcatctggcctccagtgctgccctcaacagctatgttcaattggccaacctgcccgctgatggtgcggt
cctggcccacaactatgcctgcattattaccggatggggcaaaaccagcaacaatggctctgttgcctccgtactccagcaggcccctctgcccgtggta
gcccatgcaacctgctccactggctcctattggggaagtacagtcaagtccaccatggtgtgtgctggtggtgatggagtccgttcaggatgccagggtg
attctggtggccctcttaactgccctgtcaacggtgtgtaccaagtccatggtgtcaccagctttgttgctgctgctggttgcaatacatacctgaaacc
aacagtgttcaccagagtttcagcttacatcggatggatcaatgctaatatttaaaatgtgaacgtaaaaaaagtttgaagagtaccagataactcttat
3' EST: CX970923 [+]ttattcatgtttactatcctttattagagttatctggtactcttcaaacttcttttacgttcacattttaaatattagcattgatccatccgatgtaagc
tgaaactctggtgaacactgttggtttcaggtatgtattgcaaccagcagcagcaacaaagctggtgacaccatggacttggtacacaccgttgacaggg
cagttaagagggccaccagaatcaccctggcatcctgaacggactccatcaccaccagcacacaccatggtggacttgactgtacttccccaataggagc
cagtggagcaggttgcatgggctaccacgggcagaggggcctgctggagtacggaggcaacagagccattgttgctggttttgccccatccggtaataat
gcaggcatagttgtgggccaggaccgcaccatcagcgggcaggttggccaattgaacatagctgttgagggcagcactggaggccagatgaagcactgca
atatcatacccagcagcaacattgtttctgttccagttggcatgttgcacaatccttgatactgagatgtactgttctgtgccatcattctggtaaatgt
tgtgatctcccaccaccacacgataggaagtgaccgtgtctacgcagtgagcagctgtcaggaccctgttggcacggatcagggagcccccacaggtatg
gtaccaactgccaccagacaggtactggagggaaatctgccatggccaagaattcctggctacctcacttcctccaatcactcgctgaatatcttcaatg
tagcgcacatcattgaaacaatgt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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