XB-CLONE-3556490
Clone Name: IMAGE:7713102
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Gene:
glul
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX971082 [+]
attgagtacagtcgatatcgtgggtataaagacagttgtataaagcccgtagctccagaggagccaattaacaatcccgccctccccaaagggagaaact
gaaggaatcagtcggcaccagccaatcagattccagcagggtcactcgctttgttcttatcctatagatctaaaataatcagcagccccccccaacctta
cagacaatatggtagaaccatctatacaccttagaagaattcaagtagtgtcgggagtctaacgggttaaaaaaacatggcggcgtctcgggagatgcca
tagtgattgaccaggagaagccagctcccagctcaggtctaatagtctttggtctcgctgccggtctcgttcaagatggtcgttcggaccagcgcctcgg
taacggcgtaggggtcgcagtttgctgccggccggcggtcctcgaagtaaccgtagccttcctgccccacctggcgggggatccggatgctggcaccccg
gttggcgactccggccgagaactcgtggatactggacgtctcgtgctgcccggtgagtctccgggagttgtccttcccgccccgggggtcatacacgcag
atgtggtagttgtgcctcttcccgagcttctcgatggcatcttctatgtgtttgagtcccccttcgaccctcatgctctctgtactgtagttggtatggc
aaccagcgccgttccagtttccggtcatgggcttggggtccagcgtggccaccaccccgaagtcctcgcaca
3' EST: CX971081 [+]ctcctatagggcgagaggccagtacgtaccaagcagaaggttcaaacagtgacatgtatctcgtcccagtccagatgttcagagaccccttctgcctgga
ccccaataagctggttatgtgtgaagtcttaaaatacaaccgcaagtctgcagagaccaacctccgacacacgtgtaagaagatcatggaaatggtgact
gaccatcgcccatggtttggaatggaacaggaatacaccctgctgggcattaacgggcacccgtatggttggccagagaacggcttccctgggccacaag
gtccctattactgcggcgtgggggcggataaggtgtacgggcgggacatcgtggaggcgcactacaaggcgtgtctatacgccggcatcaagatctgtgg
caccaacgcggaagtcatgccctcccagtgggagttccaggtgggaccgtgcgaaggcatcgacatgggggaccacctatggatggccaggttcatcctc
caccgggtgtgcgaggacttcggggtggtggccacgctggaccccaagcccatgaccggaaactggaacggcgctggttgccataccaactacagtacag
agagcatgagggtcgaagggggactcaaacacatagaagatgccatcgagaagctcgggaagaggcacaactaccacatctgcgtgtatgacccccgggg
cgggaaggacaactcccggagactcaccgggcagcacgagacgtccagtatccacgagttctcggccggagtcgccaaccggggtgccagcatccggatc
ccccgccaggtggggcaggaaggctacggttacttcgaggaccgccggccggcagcaaactgcgacccctacgccgttaccgaggcgctgggt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: