XB-CLONE-3556456
Clone Name: IMAGE:7712962
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Gene:
itpr3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX971106 [+]
ttttgaccagtcatctttccttcacaatgaaatggaatggcaacggaaacttcgaagcatgcctctgatatactggttttcacgtcgaatgactctttgg
ggtacaatttcattcaacctagctgttttcatcaaccttataatagcttttttctacccatatgtagagggtacatctataggaattctggattcacctt
tgatctcacttcttttctggggcatggtgtgtttctcagttatgtctttgtttacaaagcgttatggctttcggcctttgattgtggctcttatcctgcg
ctctgtgtactacctgggaattggcccaacactttacatactgggagccttaaatcttacaaacaaaattgtctttgtggtgagttttgtgggcaatcga
ggaacctttattcgtggatataaagcaatggtgatggatgtggaattcctataccatgtggcttatattctgaccagtgttcttggcctcttcattcatg
aactgttttacagcattttgctttttgatctcatctaccgagaagaaaccctgttcaatgtgataaagagtgtgacacggaatggacgctccatcttgtt
gactgccctgcttgcccttattttggtatatctcttctcaattgtgggctttctcttccttaaagatgactttattatggaggttgaccgactgcccaag
gctactagtccaatggcaccaaacaattttgctgaattctgtgagaaggaccagacagactgcactgntggtgtcacaacacttgatgagattga
3' EST: CX971105 [+]caaaatgcaaaaaacatttggttacatcttcagaaaaattataaaattcagtgtatgttaaagcccaatttgtgcagaaaaaaaatatacatttaccttg
ttttaccttgtcactttgtaaaaaatagctgcatattagcctgatacatatttattaaatacacaaattctatttttaatgaactgtattagagaaacaa
agaggtttatgtttcacaaaagtaagcacattatctattcttataaaaacactatattcatactaccattcccttttattacaatgtattttgagtgcac
acctctaaaatagtttgtacagttcactgtttatgttgtttcctagttagtgtcatatgatatgccagtatatcacaatctaacactgttagtttgttag
gtgactttaggtaatggctttaggattcattagtatttgcaccatcaccagaataacagagaaaagtctttattaatttatgatacctaccaaaacacat
aacgtaaaaatattctcaatcccttaatgtgatgatatacctttttattcatacatatgtagacctcaatagctgaagagtttaaacacacagtgcagtt
caatgttttatctatgaaaaacccagacagttctctttcccttctttttaatgattacacagaccagtatcagaaaaaaccttataatacaagtgcaact
tttccctgtcttagaaacccaaatcaaccaataagcatgtaatttttactgttcatctgaaagaaaaaaaatattataagttactaacaaactttaggac
ttttactacataac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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