XB-CLONE-3556430
Clone Name: IMAGE:7713108
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Gene:
sgk1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX971126
[+]
acattggcatgttcaactcatacacttatatcagtttaaatatacatctcttgattatatacaatatgcacaataatggtctggaatgtcaaaaaaaata
acataaataaaattgtttaaactaggcattacagccttgaacaactaaatgtggggcattttggtccagaaaaaaaaaaccaaaacatttctaaaaatag
aaattttaatagcagcaatgctttcctaaaagcattttaacaaaggtcactgcaagctcatttcaatatatatttagttagtactatctacagggaaaaa
aataactaatttctatctacattaatttagcttccacatatggaagaaatatacaatgccccaaaattagcgatcaacgcatgtcacattaacgtgtgca
aaaccacagttttactgtgataagctttacacccataccattgttcacttgtaaaaccactgtcaaaaccaaagagcccccacgtcatatggatggttct
tatgatagacaagatctctcaaaagagaaaggcacgttgcaaaagccccatactataaagacacacttggatgtcttcttccagcttaagaagccctcat
gtatccctctgtataatataagggttgtatgctgcaaattcagcaaatagttcttatttctcccaacaacagagtatgaagtccaaagtcacttttttct
atccttttctcagctccactgattcgtcccttccagcaataccagggatgttgaaattaaggctgtgatgctgttgtgaaagatgtgcaaatttcatcaa
aaggaaataaaa
3' EST: CX971125
[+]gccgctgaaatagccagtgcactaggatacttgcactctctcaacattgtttatcgtgacttgaagccagaaaacattttgttggattcccagggccaca
ttattctgactgattttggcctttgcaaggagaacatagagcccaatggcaccacatctaccttttgtggcactcccgagtatcttgcaccagaagtttt
acacaaacagccttatgacagaacagttgactggtggtgcctgggagcagtgttgtacgagatgctgtatggcctgcctcctttctacagtagaaacact
gcagaaatgtatgacaatatcttgaacaagccactgcagctaaaaccaaacataaccaactcagccaggaacctcctggagggtcttctgcagaaggacc
gaacaaaaaggattggtgcgaagaatgactttatggagattaagaaccacatgtttttctctccaattaactgggatgatctgattaataagaagattac
acctccctttaacccaaatgtgagtgggccaagtgacctgcagcactttgatccggaatttactgaagagcccgtccctaattctattggccagtctccg
gatagcatcctcataactgccagcattaaagaagctgcagaagcattcatgggcttttcttatgcaccacctatggaatcatatctttgagcaatccttc
tttctcgtctagtagtcatttctaatcaagtcactttgatttttatttccttttgatgaaatttgcacatccttcacaacagcatcacagccttaatttc
aacatccctggtattgctggaagggacgaatcagtggagctgagaaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: