XB-CLONE-3556114
Clone Name: IMAGE:7713136
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Gene:
cyp2k1.8
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX971341 [+]
gtctctgtacttctgtctgttgtcatttgcatattcctatataaagtcttctatggtggggaaaaagaatctcagaacttccctcctgggcccaaaccat
taccgatcatcggaaattttcatatgataaatatgaagaaaccccatctaacatttatggagctggctaagaagtacggctcagtattcagcattcagct
cggcccagagaaactcgttgtggtttgtggcgctgacgcagtgaaagacgctcttgtcaaccatgccgatgagttttcagcgaggcccaccattccggta
ttcgataagacttcaaagggacacggtgttttttttgctaatggtgagaactggaaagtgatgagaagattcactctttctacactaagagactttggga
tgggcaagaaaaccatagaagacaggatctgtgaagaaagtgactttttaatggaaacgttcaagtcctataaagggaagccgtttgataacaccatgat
aatgaatgcggctgtagccaacatcattgtgcacatactgctcaaccaccgctttgactatcaggatccaacgctgctcaagcttataaacatagtcagt
gagaatatcagcattgctgcaaaaccaatcgttctgctatataatgcctatccgtctattatggaatgggttcccggaactcacaaaagtgtcgctgaga
acatgttgaaactatacaactttctcagagagacatttaccccacacagggatcaactggacgtaaatgaccagagggatctcatcgatgtatttctggt
caagcaacaagaggagaaaccttcttctacaaagttctttaatga
3' EST: CX971340 [+]ctagctgcgaggcaaagcacagatctgatgctccaggggtttggatgtaattgcatcggcacaagttagatcgacctcaactccaggagggggttggaat
gtgaagttctgcagtaactttgtgaagaacaagaagagttccatcttggccaatgtctctccgacgcaaattcttcgacctgcagagaagggaaggaagg
cctcatttttcacaaaatttccttccgagtcaaggaaatgttccgggtaaaattcttctggctttttgaaataatctttatcttggagaacagatgtcag
cagtattatcacttgggttccctttggaataaaataacccctgaaggtgacgtccgtagttgtttcatggccaacattaggtgcaaggtttcctaatctc
tgaatctcatgaataacggcatcagtatagggcatttttttccgatgttcgagccgaggttgcgccgatccaatcactttgtcaatttcatcttggactt
ttttttgaatgtctggatatttcattatgaacaagaggccccagcgaagagtggtagaagtcgtctccattccggcaccaaataaatcggctaaaagtac
tgtcagattttggtcattaaagaactttgtagaagaaggtttctcctcttgttgcttgaccagaaatacatcgatgagatccctctggtcatttacgtcc
agttgatccctgtgttgggtaaatgtctctctgagaaagttgtatagtttcaacatgttctcagcgacacttttgtgagttccgggacccattccataat
agacggataggcattatatagc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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