XB-CLONE-3555991
Clone Name: IMAGE:7713435
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Gene:
eno1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX971423 [+]
aaagagtttattgcctcttcgtgtctagagtgtgacaaaccattatctagtctgaaactgcaataggatgagaacacaagactacaaagttgacatgacc
aggaccactgccacaatagaaaagacaaggactacatgcaggcaccagtgtgacactgaatttggaataatcctaagcagcaaagcttttaaactggaaa
gacagcaaggttatgctgggagaggctgtaacatctaacattgctggaagtgcctgaaattaaatgagggctcagcagtgacatgtaagacctcctgtag
tctctcctaggtgcacagctactacatcagttgggtgagagcctaaggatgcctccatttagttgaaaacaggttttctgaaatttcttccagcaaagcg
ggccttgctgccaagctcttcttcaatcctgagcaattggttgtattttgcaagtcgctcagatctgcatggagcacctgtcttgatctgtccagtgcgg
agaccaaccaccaggtcagcaatgaaagtatcttctgtctccccagagcgatgactgaccatcacaccccagccatttgactgagccagcttgcacgcct
gcagggattctgtcactgtgccgatctggttgaccttcagaagcagacagttacaagacttctcatccacagccttggcaatacgtttagggttggtcac
tgtcaagtcatcaccaactacctggatattcgaagatgcagtaaactttttccaggcttcccagtcatcctgatcaaagggatcttcaatggacaccact
ggataattcttgacaaagctcatgtancactcagccagctcgtcaggggagatgtgtcttttagggtcatcaggggac
3' EST: CX971422 [+]tttgactctcgtggtaacccaacagtggaggttgacctgtacacatgcaaaggtctcttccgtgctgctgttccaagcggagcctccactggcatttatg
aagcactagaactccgggacaatgataagactcgctacatgggaaagggtgtggctaatgcagttgagcacatcaataaaaccattgcacctgcactgat
aggcaagaatctaagtgttgtggagcaggaaaagatcgacaacctcatgattgaaatggatgggactgagaataaatccaaatttggtgctaatgcactc
ttgggagtgtccctagcagtgtgtaaagctggagctgcagagaaaggagtcccactgtaccgtcatatcgctgacctggctgggaacccagaggtcatcc
ttcctgtaccggcatttaatgtgataaacgggggttctcatgctggaaacaagctggctatgcaagaattcatgatcttgcctgtgggtgcagacacctt
taaggaggccatgcgcataggagcagaggtgtatcataacctaaagaatgtcatcaaggctaaatatggacaagatgccactaatgttggtgatgaagga
ggctttgctcccaatatcttggagaataaggaagccctggagcttctgaaaactgccatcaacaaggctggctacccagataaagttgtaattggaatgg
atgttgcagcatctgaattctaccgtgacgggaaatatgacttggacttcaagtcccctgatgaccctaaaagacacatctcccctgacgagctggctga
gttgtacatgagctttgt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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