XB-CLONE-3555988
Clone Name: IMAGE:7713483
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Gene:
maoa
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX971425 [+]
ctttctcatccaaacatataaatgtattgtgtggtcttgaatatttcaggaaaagctttataaattcagttaaaaaacatttctcccccatcccctaaaa
aagacatttacatttagttgacacaattctcagtccaatgcaaagctcagacaggcttgggggcttaattgtgatcccgtagcttcttcacactcgcgtc
ataaagccttttttgtaagcaaaagctccaacagcagcagccattccaaagaatgtagaatagccaataaacctaattaatccatgagcagatggcaggt
tacgctcccaaaatgttgtagtgattggtattgcgggaacatccacagattcaggctcagtctgccatatcttatcttctttgataagtcccatctggaa
cattatctctcttgcagctctttctccagcttgtatagccccttccatatacccactccattcagtggctgtctctgtgccagcaaaatacatttttcca
actggtttccgaataacacttccatactgagtcataattccaggagggaaatatgcagtgtaacagccaccagagtactgctctttagaccagtccttct
cttcatagtgcacaggatgtaatgcttcatcagtgcccatgactttggcatagtattcacatatttttctttttctctcttccttggttagatttgtcat
tagagttgcttttctagatagaatgaaccctattatagcaggttcagtaccatctggtttggtatcatctaatgtaaatccaattggcgtgtcatcatct
tccatgatcatgcatccacaaatatc
3' EST: CX971424 [+]cctatagggcgagaggtcttcagagacaggactgccggaactcagaagccacagccatgagcaacacatatgatgtcgtagtcgttggtggaggcatctc
aggcctttcagctgcaaaacttctgtctgagtcaggaattagtgttgtggtgttggaagctcgggaccgagttggaggaagaactcatacagtgcgaaat
aaacaggttaagtatgtagatcttggaggtgcatatgtgggaccaacccaaaatcgcttattacggatggcaaaggatctaggtcttgaaacatacaaag
taaatctcaatggccgtctcattcagcatacaaagggcaaatcatacacattttgtggagcattcccacctgcgtataatccttttgtatatttggatta
taacaatgtcatgagaacaatggaccaatgggcaaaagagatccctgcagaagctccatggaaagcacctcatgctgcagaatgggacagaatgtcaatg
atggaatttataaataatacttgttggacacagacaggaaggcgttttgcaaccatttttgtgaatttaattgttactgcagaaacccatcaaatttcta
cactttggttcttgtggtatataaagctctgcgggggaacaacaagagctttttctacagaaaatggaggtcaggagagaaaatttgttggaggctctgg
ccagataagtgaaagaatcatggaacgtctgcaaggtcgagtgatgttggagagtccagtgatcaaaattgaccagtctgggccgtgtgttatcgtggaa
actctaaccatgaaacctttgaggccaaat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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