XB-CLONE-3555718
Clone Name: IMAGE:7713317
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Gene:
amy2a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX971609 [+]
taacactcaatctggacgaacatccattgtgcacctgtttgaatggagatgggacgatattgcagcagaatgtgaacggtatttgggaccaaatggcttt
ggaggtgttcagatttcacccccaaatgaacacattattgtgacaaacccttatagaccttggtgggagaggtaccagcctataagttacaagctctgca
gtcgttctgggaatgagcaacagtttagagacatggtgacaagatgtaacaatgttggcgtatatatttacgtggatgccgtaattaaccatatgtgtgg
ctcaggtggaggtgctgggactcattcatcttgcggcagctacttcaatgcaggatccagggagttcccagttccctattctagctgggattttaatgat
ggaaagtgcaggactggcagtggtgaaatagaaaactattcggatgctaaccaggtccgagactgtcgtcttgtcggtcttcttgatttggcactggaga
aagactatgttcgtggaaagattgctgaattcatgaacaatcttattaacattggggttgctggattccgactggatgctgctaagcatatgtggcctgg
ggacctaaaggctatttcagaaagactcaataatctcaatacaaaatggttccctgctggagctcgacctttcatcttccaagaggtaattgatcttggg
ggtgaagcaatttctgtcaacgaatactatggaatcggacgagttactgagttcaagtatggtgccaagcttgggacagtcatccgcaaatggaatggag
aaaattgtcctacctaaggaactggggagaaggctggggattctgcctaagaca
3' EST: CX971608 [+]gcacttaacatagtgcaggggtatattatatgagatcatgtttttggaactttataatttggcatttacatgaatagcagcgaatgggtcttcgtcgttg
ttgcttatttggaatttagcatagccattgccatccacattgatctgcctgcctgtgcagcgagatccctccttctgtccagagatcacatcacagtagg
ttccacttggaaggccagtattcagagtgacatccaattgccaatcatcattattaaagattatgaatcctttattgccacgtccaaatgcaacttggtt
gctcccattatcccaccagtttgagaagggttgaccatctgctacattacggaaggtaaccatgttcttaatctgacgccatctatgctcacagacccag
ttgtcaccacatgttgagtcagggttgattgggactgatttggtggatccatcggaattactaggagggccaatccagtcattttgatcctttccattga
ctatgttcctagaccatctgaagctagacatgacacgagtaaaaccataaggatgtgccagcatgaagccaactcccattttgtacagacgtgcatccca
gaaggtgagtatggaagctcctccagcaccgtgtcctctctgattatcatgattgtcaacaaacaccaaggctctgtcattaggcatgaatccccagcct
tctccccagttccttaggtaggacaacttttctccattccatttgcggatgactgtcccaagcttggcaccatacttgaactcagtaactcgtccgattc
catagtattcgttgacagaaattgct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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