XB-CLONE-3555715
Clone Name: IMAGE:7713365
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Gene:
dock1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX971611 [+]
ctttactttgaacaattgtttgtattttcttagtcaggtatttataccgcactctatcagggatagcggaacgctgcctgcccctgttatcatgtacaat
agttacgtgcacgtgcttatggatacgtgtttggcagagctgaaggctgattagatatgggtctttatgaatgtaagtagataaggacactttccttgta
gataaaatcaggtataataaaaaaaaaaattaaaacaacctaaaattaaaaaaaaaaataaagaaaaaaaaatttcatatatacaaatttctgtacaagg
tgatgacttggaacttagtgaggtcatcattaggagattttgtcaaaaaacgacttagtaaatattgatattgaattattacagacgcccagcgggaggc
cctcaggctgctgtgtagagagttacagttcccagcatgctgttggggggatgctgagagatgcagaatgacagcagctggaggatgccaagctggacat
ccctcattgagtacttctctagataaattagtatccacagttcagtagctcacaaaattctcataatcatttgttaaatctaaattattttccattatcg
atgattgcacaaaaaaaaaaagaagacaccacaggccccgctgaggtaatttttgcatcattagctgaagtaagttttacatttggcatccgtgtcgaca
cagctactgctagattccaaaatccatggtggtcggctttcgcattgtctttgggggaggcggtggtggcgtcttgcttggcagc
3' EST: CX971610 [+]aaaggatggaggtttgcttccggcaactgagggagaaaatagagaagcaatacggtgtgaaaacactgtcttcaaaccctgacgagaggcgggggaatcg
gccaagatctatggtccgttcatttaccatgccgtcgtcatcgagacctttgtctgttgcctccgtgtcttcagtgtcttcagacagtaccccatcaaga
cctggctcagatgggtttgttctggaaccacttttgccaaagaaaatgcattctcgctcccaagataaattggaccgagatgatcttgataaggagaaga
aggagaaaaagaaggagaaaaggaacagcaagcaccaagagatgtttgataaagaatttaaagctactgaaattccactgcagcagtctgacgccgttat
tctgtcagaaacgataagccctttaaggcctcagcgaccaaaaagccaagttctcaacctcgttactggtgaaaggcggatttctgtatctccaggccca
gctaccccacaacaaacccagccaccacccattactccaagagttagggctctgttcagtcttccatcaggcgtggaaatgaacggtgaatcagtggcta
ccccccctccacttccagtcaaagggagcacggcagattacggcaatgtcatggatttcacggattccgtaactccttcaactcctccaccccctcctca
caggaatcttcctcctccgctgccaagcaagacgccaccaccgcctcccccaaagacaatgcgaaagccgaccaccatggattttggaatctagcagtag
ctgtgtcgacacggatgccaaatgtaaaacttacttcagctaaggatgcaaaaattacctcagcggg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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