XB-CLONE-3555653
Clone Name: IMAGE:7713657
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Gene:
znf326
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX971652 [+]
atcgattcgtttatactcgtattttgtggtgattggacaaaagacgagtaataaagttagtaatggttataacatgctgtactgtatactgtgcacagtc
ctccaagactcctacagcagatcacagcggatttactaccctaaaactgtgatgtaatgacagacataaattaaaataaaaaaggtttgtttgttttttt
tacatggtgaatataaacgctagtataaaccataaaactgaaatgtaagtgcacaacatctgaatgtggcagatcagttggtgaccagttgtaccgaagg
cagggcagacagcttatgaactggatttgttcgaaaaacatgatctctgattataaaagccagcaagactaagaggaaacatatataataatactgaaag
gaggctacagtagtcagcaggtggcaaatatgaaattcctcacccaacagtgcctagtctcagttatacaatgaaatggccctaacaatatgtacaccac
attctattcagtagcgaattataaatctcattctttctctagtacaagatggtgagagaaaattagtcaaatgattgtaatttgtatagctaaataaatg
actgaaaaaggtaaaattgaagataaatatataaaaaaagattcggtctagtctgcccattaaaatgactttgtcattgggaaaatcaaattgtgcactg
ttaaaaatgacaaagtgaaaggcatgtgtttaagggcacaatctatgctttattaagccaggacacaactgtgtccccgttttgttctctggcacatcta
ggataccatcacataatccaagccctttgtattaaatagcttaaccctcccatccctccattcctaganctaagtggaatgtccagatcaggacacaata
act
3' EST: CX971651 [+]ttgcccccagcggttgcaccttctgtgttgtatgtgtgtgagtgcatgcgtttaaccctcggtctttctgacatggtgcgttgagcctaggcagtgcact
cttcaatatttttttttcagactggataggaagccctgctgccagctgctttgcatgttggagatccattggctgtgcaaagaggaggagctagggatcc
tgtttttaatcgtggccttcttgttctaatgagcttctgtactggatcttccatacccatgccccttagcctacctatctgtctctgtattctagttctc
tatacttgataaaggattgaagccctaaagaggtttccaatgcaaagcctgtaggttttacattcctattctgatctcttatgattaatcagaatcactt
ttttcactagacagaaaggtaatggggtttagactgattcgtttaaatggaaggttatagttggtttattgtgattccagtgaagtcttctaaaaaaatt
ccttggtgctcctgaattgtcaggatccagggaaagacttaatcccttctaggacagggtgtttattaaatgctggaaataatgcattttaattttttta
tgttttaagtttgtgctgatctgatttaatgctggcattgttgttcttagccacctgactgccagcaaggtttacactttgtttcatagacaagtgccca
gtgcttggtgtatccagtgctgtttacattctttgaaaatctagatttttactttatacttcatatcccccaaaagaccagaatgcccagaagtgcctgg
gagtctagggag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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