XB-CLONE-3555650
Clone Name: IMAGE:7713323
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Gene:
cyp2f1.2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX971654 [+]
caggatccaacgctgatcaaacttataaaaagtgtcagtgagaatgtcaagattgctggaagtcctatcgtcatggtaagctgttatggggttctttcaa
cttccttccacattccccatttctctattccactgacctgtggctcatgagcaacatgttgcatgccaccccctttcatgttgctctccgtggcctcaac
agtaattttaaaattccaggtttggagccaagttttggaggcactagtttactccaagaagggtctcctgaaggctagcagtctacatggggctaccaaa
tagccaatcacagtccccaatgaactttttttcatgcttgtgtggctcctcaatgctttttccatctgattttggcttatgagtaaaataggttggggac
ccctgctctattcttttgtatagaatttttgggatcttttggtgctactgcacctcagttcagaaatactaatcctaataatgctggctggacatctttc
actgggtctccttcccatgctaagaatatctggccaggttctggttttgtgtcctcttaccagaaggattgagtaaataactttactcagctatcttcct
aatacacgtctacgtgtaccaggtaaatctgcaaggccgatttattcttccatgttctccaaggtaaacaaacatgcagagaaatgcatttattaattaa
ctgaacattttttgctgcattatttcttgaagctgtataacacatatccgtctattatgggatggattcctgngagtcacaaaactgttttcgaaaactt
ccaaaaattatccaactttcttaaagagaca
3' EST: CX971653 [+]tgcacataattcatcattacaaatacagtttacaggtaactatggttcttcgtgtagagtagaatggtttcatggcttcaccataagatgtgggagtgta
attggaatagggaaggcgtatttgtcccaaaatgttctataaagcagagaaactgaggaataacaagggctttcttcactcaggctgaaaagtcttgatc
tgtgaaaaggggtggggttttgaagagtgcggacactgaccaggacttcaagaaatagctaaaattacaagtaaataaaggttttggtttcttaagactg
tttatagctttctatgtttgatttttgtaaatgagaagctccattttatggtgatgatgtgtagagtttgtctagctgcgaggcaatgcacagatctgat
ggtctaaaggtatggatgtgagtgcatctccacaggtcagttggacctcaactccaggagggggttggaatgtgaagttctgcagaagttttgtgaagaa
caagaagagttccatcttggccaacgtctctccaacacaacttcttttacctactgagaagggaaggaaggcctcatttttcacaaaatttccttttgag
tcaaggaaatgttccgggtaaaattcttctggtttttggaaataatctttatcttgcataacagaaatcagcagggggatcacttgggttccctttggaa
ggaaatagcctctgaatgtgacgtcctcagttatcgaatggggaaggacaataggcacgaggttggcaaatctctgaatctcatggataacggcatcggt
atagggtatttgtt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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