XB-CLONE-3555647
Clone Name: IMAGE:7713371
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Gene:
apoe
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX971656 [+]
caggaggatttaaaaaagaagctcagccaatatggagaagatttccgtaagagtactgaagacaaagtggaaaatctccgtaaagccgtggagccttatt
tgtccaacgtgaaagataaagggcagcagaggctgatgaacctgaaggatgccatagatgagcaagggaaacaactcagagaccggtttgtctctgttgc
caaagacgcccaagctaaaatgaagaagacagcctctgatgtgaaaagcagtgtggataaactaggggaacagttccgtaaatggttcaaccccaatgta
gagagcctgcgcaatcagcttcaagccttggctaagagcctgcagaccaaggccaacaaataaatgaaagaaccagtgtttttccttgcttctcacttgc
agctgctacttttgaccataagattgcgccctgaggtggagcaggacattaaggatccaacatgcctaaacatcttgcctttccctgtttgtatctgagg
tacatcacccagtgattatactgctcacctaggcaagctcattaatcagatctctgacattaatgacaagcacaaattagaggtgaacgatgtaaagcca
aagggaacttcagtattaaaggcacatttaacctaagggcaagccttaaattatagcactaaactatccccagcatattctcctgcactaaacatgcctt
gtgtcctacagatctccagctgactgccctgcctgaacaagaacctacataccttaggcaagttaagattcacagaanagctaagacagaaaagtatatg
agcctctacatgttatatttttcatttggaacacaggcccccaggggcagcagtaaactaggccatgcaaggaatcacttgtaccaacagctggggtctt
aaaca
3' EST: CX971655 [+]tacagcagagaggcactttataaagtaggtaaggcagcacatacagcagaaatctagtgaaggcacaacgttattttaggtttaaaaaaggggagtgtat
taagaaacataaagacaccagcaatgcccataatggtgccctacattttcaagagctggtgatacaatatggatatttagtaggttgagatacagacatc
catgaaccctcttgtttaagaccccagctgtttggtacaagtgattccttgcatggcctagtttactgcttgcccttggggcctgtgttccaaatggaaa
atataacatgtagaggctcatatacttttctgtcttagcttttctgtgaatcttaacttgcctaaggtatgtaggttcttgttcaggcagggcagtcagc
tggagatctgtaggacacaaggcatgtttagtgcaggagaatatgctggggatagtttagtgctataatttaaggcttgcccttaggttaaatgtgcctt
taatactgaagttccctttggctttacatcgttcacctctaatttgtgcttgtcattaatgtcagagatctgattaatgagcttgcctaggtgagcagta
taatcactgggtgatgtacctcagatacaaacagggaaaggcaagatgtttaggcatgttggatccttaatgtcctgctccacctcagggcgcaatctta
tggtcaaaagtagcagctgcaagtgagaagcaaggaaaaacactggttctttcatttatttgttggccttggtctgcaggctctta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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