XB-CLONE-3555643
Clone Name: IMAGE:7713467
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Gene:
dcn
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX971660 [+]
gaacagtaacaggtgtgcctcatttaagggagcttcacttagaccataattccctgacacaggtgcctgctgggcttggcgaacacaagtacattcaggt
tgtgtaccttcataacaataaaatttctgctgtctccacgaatgatttctgcccacttggatataacacaaagaaggcctcatactcaggcatcagcctt
tttagcaatccagtgcaatattgggaaattcagccggccacattcagatgtgtatatgaacgatctgctatacaaattggaaactacaagtaaatcatgg
acgtgttgcaaataaagtcttttttttgtttgttttctcttctttgtttgtttgtttaaatacattcttagtgaataaaaatgtccaatactggaaagta
tcccaaaacagaatagccagcttactatcatgagacaggagttttctgcattattttgttttgagttgcataatgatgaataataaagacatttagctgt
gctgttctgttcccttattaagtttattgtatactgttagtattatacccacagaaaagttgatccaaataatctgtacttttcaaaaaaataaattgta
gtagtattggataatcattaggccaaacaatgttcgtccaaatcacaaaaacttgtaactacaactaaattacattttaatgaatggtaatactgagtgg
tacacacacacacacacaattttcagaataggttataccaggagtccccaacctttgaaactcgcgagccacattcaaataaaaaagtgttgggaaacaa
aacaagtatgaaaaaagtttctggggg
3' EST: CX971659 [+]ttgcctcataaagctcctcagctcaagatcagtttaggccgaggaggtcggggcgctggtgtgcaatgcatgttcgtctggcttacatgatcctgcggca
ccaggagagaattaagctcgcacatggcactccccacaagccacccttccggagtgatctcctctcctgtctccctgcaggactcagttctctgccatcc
tcacatgaactggcacgaacctcttccctcttcaccggctcccctggacattttcaggtctctcctcaagctgcagccccctttcttcccccttctggac
ctgtggatccattcataaggacgtctgggttttctcctctggctgcccttaataatggtgcatttggaggactagggaacccatcgtacaatcctcccac
aatgtttaaccggaaggatggccctgcaattcagggctttcccaacccccatgacccatggaatcggctccaccgcacacctccatcttttccgacagca
ccgcctcagatttgtgccaagcctgatctggatgcacattctgtgccactgatgaagaatgagaaggatcgggatcttttctgccgcctggcaccacata
tctcccctctgacctccagccagcgcctccctggtggggaggagcgtgggccgtcgcgtacagtctctccgtacatggcagcaaggcatggaagcagttc
tgacatcaagcctcgcctgacgcctgatcactccaaggtgaaggtgaaggaggaacccgaggtgctgacatttgaagtggggcgctctcctgtgcctggg
ctacacttgtcgcatccgtctcccccctttgagaggc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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