XB-CLONE-3555613
Clone Name: IMAGE:7713565
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Gene:
commd9
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX971680 [+]
cttcagcctcccccaaatgcacagcagtaggacaagcggcagagacatctgcattactcctaggcttccagagaggccagacagacactgcttcccggca
tcagctgatctatagatataaaggcaggttctgctatataatgtgggtcaacagtgtccagcctatagccttccctagtggatatatgcggactgctggg
aatttgttaaagggttggaggactgcaggtacatccctgatggtaaatggggggttatgtaataaatggattactgctcctgggcaaacttagtgccttt
tattataaatgggggataatgttttgtatatgttttacagtacagtttttttttttattgcgccataatatttaacatcggggctgtcctcattttgata
aaagctccaaacttcatgatataattcatggattgtgcgatgtagccgcccgtctcttcccgacacatctctgcccatgatttaaatggagctgcactta
attaataaaccgttattccgaaaacttgcagggaattatcctgtgctgaatatgcctctatttatccaccagggggtgctgtcttgcttttgaaatttta
gcctgaagcctagttatagtgcgttcccgtagatggtaaataataatcaatattttaggcttaattaccctgaagataaagggatttctttttctgtgac
catttataatgaataattgccacaagccgactggaagatggaactgtattgtgagaactgcggagtataattagtaagacccctggggaataagtggata
aataaggaaaagtaaaacaaaatattgtcctttattt
3' EST: CX971679 [+]ccttctattttatgcacccaactaaaataaacccaaggcacagagacaggcaggcagatttagattttgtctcaatacaggattcacacattgggtgact
tgttttactgatatctgtataaaggagatataaacccaaaaataccattttcccaaaggaaagaaaatagaattctaagcaattatacatcataaccatt
cattatacagggcacttgttattctctgtatccactgctggttctgactactgaaacgatgcagcagaagccagtccagctccagttcctacaatgcctt
gccagctggcttctgctgcactgttcaattgaacagcttcactgatccagcccaaaatgcaaaggggcagctgggaatgaagacatatcagtcatagaaa
tgctgtaccccttcagcacaaggaagcacagtacaggagatagagcaagggcaatacatgtaatcaacctaccagcacacagcatgatcacatgtatact
gtggaaataaaggacaatattttgttttacttttccttatttatccacttattccccaggggtcttactaattatactccgcagttctcacaatacagtt
ccatcttccagtcggcttgtggcaattattcattataaatggtcacagaaaaagaaatccctttatcttcagggtaattaagcctaaaatattgattatt
atttaccatctacgggaacgcactataactaggcttcaggctaaaatttcaaaagcaagacagcaccccctggtggataaatagaggcatattcagcaca
ggataattccctngcagttttcgga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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