XB-CLONE-3555582
Clone Name: IMAGE:7713377
|
Gene:
rnpep
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX971702 [+]
cgtagcgggacaccatgtcagccagaggcaggacggcggaagatgtagccacagcttccagttaccgggagttccagcccgagcacatgcacctggagct
gacagtagacacagaggctcgaatactgcggggctcccagcggctggacgtgcaggtgttgcgggagaccgaggagctgcgactggacactcaccgctgc
ttgcgagtgctgggagctcggctggagaagggccagggggaacatgaggagttgtcgtgggaggaaaagcctttcacacgctatggcactatggtggttc
tccgtctgcccagcctactgccccccggagccagggttcgggtgatactcgattatgaggcagcggatagcccaggggtttgttggttggacccacagca
gactgcaggcaagaagaagccttacatgtacacgcaaggccaggctgttttgaaccgctccttcttcccatgctttgacacaccggctgttaaatgcacc
tattctgccaatgtcaaggtgcctgaaggttttacagctgttatgagtgcaaataactctgaccgccaaggggatacttttaaattcgagatggcacaac
ctattccagcatatctggtggctctggtggttggtgacattgtatcagcagaggtaggaccaaggagccgtgtgtgggccgagccttgtcttatagaagc
agctaaaaaggagtatgatggagtaatagaggagttcctgaaagtgggggagaagctctttggtccttatgtatggggaaggtatgatgtactattcatg
ccaccatctttcccatttggtggaatggagaacccctgcatcacatttgttaccccatgtctgctggctggtgatcgctcattggctgatgttatcata
3' EST: CX971701 [+]gtagctggacaatgatctgcaatgctctatatgcgctctcattgtgcagatgtgcaactctatgttcctaggatcctctttacatagttctgcacattgg
catgaagctgtggacaagtttgcgtaaaggtctccctagcgaggtcctgtgctgcctttgagccagcttgcatggcacggtataaaggtaaagtatactt
ctgcttcccctggcaatggagaaagtccctgactttatgaaagtggagctgatagtcattcttcaatacaatctgggcccagcgcaaacgcagctcagca
tttgtagcattggaaatctttggataatacttttccagttgttcgacatttccatcaggcagtggtgacaactcaagtactctatccaaaaagtagacaa
gctgataggtcctccatattgtgggatcaacttttgaaatagtttcagtatcaagtggagtagaagaccagaggtttgctagttctgttgctggtttcat
aagtgcctctccaggggacaggtctgggaggaaaggaggccatcctggagtgtttagccaatggtcaaattccagaccggatatcgtatccactcccttt
gctttcagttctggaaaatattctagataaaactcaagggcttcatcagcaagaatgctctggaacttgaacttattcacataggcctttaaaaatgcat
caaacttggtctggtccccagtgaggtgggccaagtatgaaacaaagcagaaccctttctcgtagggagtctcattataagtatcatctggatccacacc
aggctctatctttactcgtaatttgttcaaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: