XB-CLONE-3555579
Clone Name: IMAGE:7713425
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Gene:
clk3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX971704 [+]
tgagcctcatgtgtgaacaatgcagggattaaaaggtgtgaacagtacaggggattacacatttaaacaatacgtgggaattgtagcctaaatctgaggt
gtgagcaatgcagggggccagataatctcagtactgataccatttaaaacttacacaaaggtaagcagtcacagcagccagacaggaagggggccacaca
gaggggggtcgtgggccgccagatggacagcactgctataaagaattcatttacagtgctgaggtcctgactttgcatccaaccaagggaactttaaggc
tgaagttctccttgtgattgagagggtttcctctgggtgtgccacaaatcacaatgcaaacatttccatatgtgcttatggctgtcctctacccatggct
cctcatgaaaatttcatgcatagttagaccccatagttaaccctttaacaattataaatggaatacatagtgtgctagcaagttgggactgccttttgta
tagaccagccagcaatcaaccccaaaaaacattgtttgagcttccagcttttgagtctgtgagttttgttagagagttagatttatttcttgcacacaga
cccccaaagtttatactggtattagatataaatagtggcttatctcttatcttgaagtctatcagctaagttggcagcactatacatgatcaatgtgctc
tggggtgataatagtaaataataggcattgttcatcgcaccatgttattaatgtgtaaaggggttccagtacacccaggctaatagagcagccctgttcc
agtcttagcatatttctatcatattgtgacttaaggtttgcacacacgatatggccatttgtcttttattt
3' EST: CX971703 [+]tttttttttatgaaaacaaatcaccttcacaaatttattgatagaacaaactttcagtaacaaagacagggaaactattaagatcctccctataatataa
gtggcacaccagtaagagggaagtgtgctttgtaatattctggtttagaccttgcacctccttccccactgaaattcagctgagtacttgctcagtgctc
tacctccctgagtgcacccatatatacatttgtatgaactattcacctctaataaatagaaaagcaacatttgtgttacatggctgcagcttcccacaat
gctctgcactaaaactgacagtaggactcagacgatgaactataagtatccaccatgatcacaggtacgcagagagatcacattgttcttaatggctctt
gcaccataatcaagcaaattgacaaacgcttgaagtgtgcaacttctatccttgattgctgttgactaaattgtgcatgtgtgtgtagcactggtcctct
taaacaaagaaaacaatattacagatatattgggaaatatgttcagagcaagaagcgtgccacagtgatgatacaatcagtccgaatgacagcaatatac
tcgctaaagtgtccagtcaaactctgttcgtgtcatctgctgaggtctcttgagattcggttacccagagcattctcttctggcttcaagcaagtgaaaa
attgatgttccagggcttcctgaagcgtgatgcgcagcgcaggtctgaactccagcatcctgctcagcaagtcaaacagctgcacgtgttccgcagaatc
actgctcttgtaactctagcaggaacccaaacataaaacatcaatgttagattcaggcatggaaacactgagaaggggtttct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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